Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24728 | 5' | -52.9 | NC_005264.1 | + | 147476 | 0.66 | 0.96658 |
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Target: 5'- gCGGCACcgG-GGCUaguguguggCAGAGGUUCc- -3' miRNA: 3'- -GCCGUGuaCaCCGAa--------GUCUUCGAGaa -5' |
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| 24728 | 5' | -52.9 | NC_005264.1 | + | 106443 | 0.67 | 0.951561 |
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Target: 5'- -uGCACGaGcGGCUUCGGAGGCgcgCUg -3' miRNA: 3'- gcCGUGUaCaCCGAAGUCUUCGa--GAa -5' |
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| 24728 | 5' | -52.9 | NC_005264.1 | + | 8446 | 0.68 | 0.90308 |
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Target: 5'- cCGGCGCAacUGGUUUCAGcucGAGUUCg- -3' miRNA: 3'- -GCCGUGUacACCGAAGUC---UUCGAGaa -5' |
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| 24728 | 5' | -52.9 | NC_005264.1 | + | 127473 | 0.68 | 0.90308 |
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Target: 5'- cCGGCGCAacUGGUUUCAGcucGAGUUCg- -3' miRNA: 3'- -GCCGUGUacACCGAAGUC---UUCGAGaa -5' |
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| 24728 | 5' | -52.9 | NC_005264.1 | + | 60729 | 0.7 | 0.843071 |
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Target: 5'- uGGCGCAUGUGGauggUAGAGGC-Cg- -3' miRNA: 3'- gCCGUGUACACCgaa-GUCUUCGaGaa -5' |
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| 24728 | 5' | -52.9 | NC_005264.1 | + | 125882 | 0.7 | 0.81697 |
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Target: 5'- uGGUGCAuuaUGUGGCUUgCGGAAGCg--- -3' miRNA: 3'- gCCGUGU---ACACCGAA-GUCUUCGagaa -5' |
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| 24728 | 5' | -52.9 | NC_005264.1 | + | 29608 | 0.73 | 0.698902 |
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Target: 5'- uCGGCGCu--UGGCUUCGaAAGCUCUa -3' miRNA: 3'- -GCCGUGuacACCGAAGUcUUCGAGAa -5' |
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| 24728 | 5' | -52.9 | NC_005264.1 | + | 109350 | 0.75 | 0.561448 |
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Target: 5'- cCGGCGCAUGgGGCUUCuaugguggGGGAGUUCg- -3' miRNA: 3'- -GCCGUGUACaCCGAAG--------UCUUCGAGaa -5' |
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| 24728 | 5' | -52.9 | NC_005264.1 | + | 137902 | 1.07 | 0.006388 |
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Target: 5'- aCGGCACAUGUGGCUUCAGAAGCUCUUg -3' miRNA: 3'- -GCCGUGUACACCGAAGUCUUCGAGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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