Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24737 | 3' | -50.3 | NC_005264.1 | + | 99761 | 0.66 | 0.997142 |
Target: 5'- uCGCCCgGCgagGagaccauacgggacuUCUUGUUUGGGaACGc -3' miRNA: 3'- uGCGGG-CGaa-U---------------AGAACAAACCCaUGC- -5' |
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24737 | 3' | -50.3 | NC_005264.1 | + | 94265 | 0.66 | 0.996944 |
Target: 5'- -gGCCUGCUUAUCUUuccaggaGGGUuCGg -3' miRNA: 3'- ugCGGGCGAAUAGAAcaaa---CCCAuGC- -5' |
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24737 | 3' | -50.3 | NC_005264.1 | + | 56077 | 0.67 | 0.993348 |
Target: 5'- gACGCCCGCUgacgccgcGUCUUGUgc--GUGCc -3' miRNA: 3'- -UGCGGGCGAa-------UAGAACAaaccCAUGc -5' |
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24737 | 3' | -50.3 | NC_005264.1 | + | 108483 | 0.67 | 0.991068 |
Target: 5'- uCGUCCGCUUcgCUgauccucucuagugGGGUACGg -3' miRNA: 3'- uGCGGGCGAAuaGAacaaa---------CCCAUGC- -5' |
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24737 | 3' | -50.3 | NC_005264.1 | + | 68174 | 0.68 | 0.986957 |
Target: 5'- gGCGCCCGCcgcggCUacGUUUGGGcUugGu -3' miRNA: 3'- -UGCGGGCGaaua-GAa-CAAACCC-AugC- -5' |
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24737 | 3' | -50.3 | NC_005264.1 | + | 20539 | 0.69 | 0.964819 |
Target: 5'- uGCGCCCGUUgcauggCUUGUaugUGGGgAUGg -3' miRNA: 3'- -UGCGGGCGAaua---GAACAa--ACCCaUGC- -5' |
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24737 | 3' | -50.3 | NC_005264.1 | + | 143989 | 0.71 | 0.924369 |
Target: 5'- gGCGCUCGUaacAUgUUGcUUGGGUACGg -3' miRNA: 3'- -UGCGGGCGaa-UAgAACaAACCCAUGC- -5' |
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24737 | 3' | -50.3 | NC_005264.1 | + | 143829 | 1.08 | 0.01166 |
Target: 5'- cACGCCCGCUUAUCUUGUUUGGGUACGc -3' miRNA: 3'- -UGCGGGCGAAUAGAACAAACCCAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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