Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24738 | 3' | -48.7 | NC_005264.1 | + | 30561 | 0.66 | 0.99935 |
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Target: 5'- cGUCGUCcucCGAGucGGUAGAGGaccaccugcuucuCGGGa -3' miRNA: 3'- -CAGCAGaa-GCUCuuUCAUCUCU-------------GCCU- -5' |
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| 24738 | 3' | -48.7 | NC_005264.1 | + | 149588 | 0.66 | 0.99935 |
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Target: 5'- cGUCGUCcucCGAGucGGUAGAGGaccaccugcuucuCGGGa -3' miRNA: 3'- -CAGCAGaa-GCUCuuUCAUCUCU-------------GCCU- -5' |
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| 24738 | 3' | -48.7 | NC_005264.1 | + | 31525 | 0.66 | 0.999215 |
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Target: 5'- cUCGgg--CGGGGAgggccguuggGGUGGGGGCGGAc -3' miRNA: 3'- cAGCagaaGCUCUU----------UCAUCUCUGCCU- -5' |
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| 24738 | 3' | -48.7 | NC_005264.1 | + | 150552 | 0.66 | 0.999215 |
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Target: 5'- cUCGgg--CGGGGAgggccguuggGGUGGGGGCGGAc -3' miRNA: 3'- cAGCagaaGCUCUU----------UCAUCUCUGCCU- -5' |
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| 24738 | 3' | -48.7 | NC_005264.1 | + | 27609 | 0.67 | 0.998826 |
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Target: 5'- cUCGUUUUCGAGguAGaUGGcuAGGCGGc -3' miRNA: 3'- cAGCAGAAGCUCuuUC-AUC--UCUGCCu -5' |
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| 24738 | 3' | -48.7 | NC_005264.1 | + | 15329 | 0.67 | 0.998577 |
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Target: 5'- --aGUCUUCGGGAAacguGGUAGAG-CaGAu -3' miRNA: 3'- cagCAGAAGCUCUU----UCAUCUCuGcCU- -5' |
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| 24738 | 3' | -48.7 | NC_005264.1 | + | 9664 | 0.71 | 0.974609 |
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Target: 5'- aUCGUCUagCGAGGccuuGGgGGAGGCGGGc -3' miRNA: 3'- cAGCAGAa-GCUCUu---UCaUCUCUGCCU- -5' |
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| 24738 | 3' | -48.7 | NC_005264.1 | + | 128691 | 0.71 | 0.974609 |
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Target: 5'- aUCGUCUagCGAGGccuuGGgGGAGGCGGGc -3' miRNA: 3'- cAGCAGAa-GCUCUu---UCaUCUCUGCCU- -5' |
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| 24738 | 3' | -48.7 | NC_005264.1 | + | 144061 | 1.1 | 0.014883 |
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Target: 5'- gGUCGUCUUCGAGAAAGUAGAGACGGAa -3' miRNA: 3'- -CAGCAGAAGCUCUUUCAUCUCUGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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