Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24743 | 5' | -55.4 | NC_005264.1 | + | 76214 | 0.66 | 0.923279 |
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Target: 5'- uGGUCGGUgcugcGGCGGUUgaucaacguagcgcuUGGGUgUGGAGc -3' miRNA: 3'- gCCAGCCG-----CCGCUAG---------------ACUCAaACCUU- -5' |
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| 24743 | 5' | -55.4 | NC_005264.1 | + | 135068 | 0.67 | 0.890109 |
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Target: 5'- uGGUCGGCGGCcAUCcgUGGGaagacgGGAu -3' miRNA: 3'- gCCAGCCGCCGcUAG--ACUCaaa---CCUu -5' |
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| 24743 | 5' | -55.4 | NC_005264.1 | + | 30394 | 0.69 | 0.811815 |
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Target: 5'- uCGG-CGGCGGCGGUCgcgcgccGGUgcGGAGa -3' miRNA: 3'- -GCCaGCCGCCGCUAGac-----UCAaaCCUU- -5' |
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| 24743 | 5' | -55.4 | NC_005264.1 | + | 149421 | 0.69 | 0.811815 |
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Target: 5'- uCGG-CGGCGGCGGUCgcgcgccGGUgcGGAGa -3' miRNA: 3'- -GCCaGCCGCCGCUAGac-----UCAaaCCUU- -5' |
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| 24743 | 5' | -55.4 | NC_005264.1 | + | 56819 | 0.7 | 0.746861 |
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Target: 5'- uCGGUCGGCGGCGcaugaucaucGUUUGAGUc-GGu- -3' miRNA: 3'- -GCCAGCCGCCGC----------UAGACUCAaaCCuu -5' |
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| 24743 | 5' | -55.4 | NC_005264.1 | + | 43474 | 0.72 | 0.640087 |
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Target: 5'- aCGGUUGGCGGUugucgccauugccgcGAUUUGGGgggUGGGAg -3' miRNA: 3'- -GCCAGCCGCCG---------------CUAGACUCaa-ACCUU- -5' |
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| 24743 | 5' | -55.4 | NC_005264.1 | + | 162501 | 0.72 | 0.640087 |
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Target: 5'- aCGGUUGGCGGUugucgccauugccgcGAUUUGGGgggUGGGAg -3' miRNA: 3'- -GCCAGCCGCCG---------------CUAGACUCaa-ACCUU- -5' |
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| 24743 | 5' | -55.4 | NC_005264.1 | + | 146950 | 1.06 | 0.005296 |
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Target: 5'- cCGGUCGGCGGCGAUCUGAGUUUGGAAc -3' miRNA: 3'- -GCCAGCCGCCGCUAGACUCAAACCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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