Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24755 | 3' | -56.5 | NC_005264.1 | + | 80746 | 0.66 | 0.917129 |
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Target: 5'- ------cGCGGAucguGGGGACaAUCCUCGGc -3' miRNA: 3'- cacaacaCGUCU----CCCCUG-UGGGAGCC- -5' |
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| 24755 | 3' | -56.5 | NC_005264.1 | + | 31736 | 0.66 | 0.885852 |
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Target: 5'- -cGUcGUGUaAGAGcaGGGGCGCCUUUGGc -3' miRNA: 3'- caCAaCACG-UCUC--CCCUGUGGGAGCC- -5' |
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| 24755 | 3' | -56.5 | NC_005264.1 | + | 150762 | 0.66 | 0.885852 |
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Target: 5'- -cGUcGUGUaAGAGcaGGGGCGCCUUUGGc -3' miRNA: 3'- caCAaCACG-UCUC--CCCUGUGGGAGCC- -5' |
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| 24755 | 3' | -56.5 | NC_005264.1 | + | 86649 | 0.67 | 0.85687 |
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Target: 5'- -cGUUGgGCGccgcGGGGGACACCCUa-- -3' miRNA: 3'- caCAACaCGUc---UCCCCUGUGGGAgcc -5' |
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| 24755 | 3' | -56.5 | NC_005264.1 | + | 126273 | 0.68 | 0.833027 |
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Target: 5'- cGUGUUGguccGCGGAGGcGGccaugcCGCUUUCGGg -3' miRNA: 3'- -CACAACa---CGUCUCC-CCu-----GUGGGAGCC- -5' |
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| 24755 | 3' | -56.5 | NC_005264.1 | + | 16374 | 0.69 | 0.765912 |
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Target: 5'- aGUGcUGggGgGGAGGGGAUGCCCguggcgcauuggcacUCGGg -3' miRNA: 3'- -CACaACa-CgUCUCCCCUGUGGG---------------AGCC- -5' |
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| 24755 | 3' | -56.5 | NC_005264.1 | + | 105142 | 0.69 | 0.76122 |
|
Target: 5'- -gGgaGcUGCAG-GGGGAggcaacgaaggucCACCCUCGGg -3' miRNA: 3'- caCaaC-ACGUCuCCCCU-------------GUGGGAGCC- -5' |
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| 24755 | 3' | -56.5 | NC_005264.1 | + | 114281 | 0.7 | 0.683093 |
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Target: 5'- aUGUUGUGCGGAcgacgaaggcgacGGGGGCGCg--CGGg -3' miRNA: 3'- cACAACACGUCU-------------CCCCUGUGggaGCC- -5' |
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| 24755 | 3' | -56.5 | NC_005264.1 | + | 158721 | 0.95 | 0.02619 |
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Target: 5'- cGUGUUugGCAGAGGGGACACCCUCGGc -3' miRNA: 3'- -CACAAcaCGUCUCCCCUGUGGGAGCC- -5' |
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| 24755 | 3' | -56.5 | NC_005264.1 | + | 39695 | 0.95 | 0.02619 |
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Target: 5'- cGUGUUugGCAGAGGGGACACCCUCGGc -3' miRNA: 3'- -CACAAcaCGUCUCCCCUGUGGGAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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