Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
24760 | 5' | -57.3 | NC_005264.1 | + | 136929 | 0.66 | 0.88083 |
Target: 5'- aCAGCGcCCCCCGccaUCUguucgccUGGGGcaUCGCa -3' miRNA: 3'- -GUCGUuGGGGGC---AGA-------GCCCUucAGCG- -5' |
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24760 | 5' | -57.3 | NC_005264.1 | + | 67739 | 0.66 | 0.874526 |
Target: 5'- uGGC-GCCUUCGcguaUCUCGGcGcAGGUCGCa -3' miRNA: 3'- gUCGuUGGGGGC----AGAGCC-C-UUCAGCG- -5' |
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24760 | 5' | -57.3 | NC_005264.1 | + | 46146 | 0.66 | 0.874526 |
Target: 5'- gGGCAACCCUCGU---GGcGAAGUCa- -3' miRNA: 3'- gUCGUUGGGGGCAgagCC-CUUCAGcg -5' |
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24760 | 5' | -57.3 | NC_005264.1 | + | 6103 | 0.66 | 0.867324 |
Target: 5'- aCGGCGGCCauguCUGUUUUGuGGAAGguguUCGCg -3' miRNA: 3'- -GUCGUUGGg---GGCAGAGC-CCUUC----AGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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