miRNA: 3'- guCGUUGGGGGCAGa--GC--------------CCuuC-AGCG- -5'
miRNA: 3'- guCGUUG--GGGGC-AgAGCCCUU-----CAGCG- -5'
miRNA: 3'- -GUCGUUGGGGGCAGAGCCCUUCAGCG- -5'
miRNA: 3'- -GUCGUUGGGgGCaGaGCCCU----------UCAGCG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24760 | 5' | -57.3 | NC_005264.1 | + | 156495 | 0.69 | 0.735813 |
|
Target: 5'- -uGCAGCCCCUGUCccgCGuaucgcacagcuacGGggGcUCGCg -3' miRNA: 3'- guCGUUGGGGGCAGa--GC--------------CCuuC-AGCG- -5' |
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| 24760 | 5' | -57.3 | NC_005264.1 | + | 158773 | 0.69 | 0.748197 |
|
Target: 5'- -uGCAACagCCCCGcUgUCGGGAGaucccGUCGCc -3' miRNA: 3'- guCGUUG--GGGGC-AgAGCCCUU-----CAGCG- -5' |
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| 24760 | 5' | -57.3 | NC_005264.1 | + | 160307 | 1.1 | 0.002042 |
|
Target: 5'- aCAGCAACCCCCGUCUCGGGAAGUCGCc -3' miRNA: 3'- -GUCGUUGGGGGCAGAGCCCUUCAGCG- -5' |
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| 24760 | 5' | -57.3 | NC_005264.1 | + | 161939 | 0.68 | 0.802917 |
|
Target: 5'- cCAGCGGCCCgCGaCaCGGGGccguacacccAGUCGUa -3' miRNA: 3'- -GUCGUUGGGgGCaGaGCCCU----------UCAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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