Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24762 | 3' | -53.6 | NC_005264.1 | + | 146235 | 0.72 | 0.733638 |
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Target: 5'- cGGCGUcguGGCGCCCGGAAuacaacuucacuuacGAG-UGGUAc -3' miRNA: 3'- -CCGCA---UUGCGGGCCUUu--------------CUCuACCGU- -5' |
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| 24762 | 3' | -53.6 | NC_005264.1 | + | 101448 | 0.73 | 0.712677 |
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Target: 5'- cGGCGaaggAGCGCCUGGAAcggGGAGAccGCGa -3' miRNA: 3'- -CCGCa---UUGCGGGCCUU---UCUCUacCGU- -5' |
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| 24762 | 3' | -53.6 | NC_005264.1 | + | 148512 | 0.74 | 0.651383 |
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Target: 5'- uGGCGgcgAGCGCCgCGGAAGaaauuuguGuGAUGGCGu -3' miRNA: 3'- -CCGCa--UUGCGG-GCCUUU--------CuCUACCGU- -5' |
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| 24762 | 3' | -53.6 | NC_005264.1 | + | 161192 | 1.11 | 0.003937 |
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Target: 5'- uGGCGUAACGCCCGGAAAGAGAUGGCAg -3' miRNA: 3'- -CCGCAUUGCGGGCCUUUCUCUACCGU- -5' |
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| 24762 | 3' | -53.6 | NC_005264.1 | + | 42165 | 1.11 | 0.003937 |
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Target: 5'- uGGCGUAACGCCCGGAAAGAGAUGGCAg -3' miRNA: 3'- -CCGCAUUGCGGGCCUUUCUCUACCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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