Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24795 | 3' | -45.1 | NC_005282.1 | + | 74656 | 0.66 | 0.99956 |
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Target: 5'- -uUCACUaa-GUAuCCUGAA-GGCUGg -3' miRNA: 3'- guAGUGAaugUAUuGGACUUuCCGAC- -5' |
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| 24795 | 3' | -45.1 | NC_005282.1 | + | 40138 | 0.67 | 0.999293 |
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Target: 5'- aCAUCAUUUGCAcUGGCUaauucuguaccuUGuAGGGCUGu -3' miRNA: 3'- -GUAGUGAAUGU-AUUGG------------ACuUUCCGAC- -5' |
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| 24795 | 3' | -45.1 | NC_005282.1 | + | 77796 | 0.67 | 0.998897 |
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Target: 5'- aCGUCACca--AUAACCUGuAAGGCc- -3' miRNA: 3'- -GUAGUGaaugUAUUGGACuUUCCGac -5' |
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| 24795 | 3' | -45.1 | NC_005282.1 | + | 33737 | 0.68 | 0.998326 |
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Target: 5'- uCGUUACUgACAaauuAUCUGuAGAGGCUGg -3' miRNA: 3'- -GUAGUGAaUGUau--UGGAC-UUUCCGAC- -5' |
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| 24795 | 3' | -45.1 | NC_005282.1 | + | 54116 | 0.68 | 0.997957 |
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Target: 5'- -cUCAgUa--AUAGCUUGGAGGGCUGa -3' miRNA: 3'- guAGUgAaugUAUUGGACUUUCCGAC- -5' |
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| 24795 | 3' | -45.1 | NC_005282.1 | + | 33888 | 1.11 | 0.013751 |
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Target: 5'- cCAUCACUUACAUAACCUGAAAGGCUGa -3' miRNA: 3'- -GUAGUGAAUGUAUUGGACUUUCCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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