Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24796 | 5' | -41.5 | NC_005282.1 | + | 66962 | 0.66 | 1 |
Target: 5'- --cAGCACCAUCUU--CAGUgGGUa- -3' miRNA: 3'- uaaUUGUGGUAGAAuuGUUAgUCAgu -5' |
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24796 | 5' | -41.5 | NC_005282.1 | + | 38988 | 0.66 | 1 |
Target: 5'- ---uGCAgCAUCUUGACAAUauaCAGUg- -3' miRNA: 3'- uaauUGUgGUAGAAUUGUUA---GUCAgu -5' |
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24796 | 5' | -41.5 | NC_005282.1 | + | 51375 | 0.66 | 1 |
Target: 5'- cUUAcCACCAUaaaguaaCUUAACAAUCccuaAGUCAc -3' miRNA: 3'- uAAUuGUGGUA-------GAAUUGUUAG----UCAGU- -5' |
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24796 | 5' | -41.5 | NC_005282.1 | + | 2460 | 0.66 | 0.999999 |
Target: 5'- uUUAACACC-UCaacccguuCAGUCGGUCGu -3' miRNA: 3'- uAAUUGUGGuAGaauu----GUUAGUCAGU- -5' |
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24796 | 5' | -41.5 | NC_005282.1 | + | 47847 | 0.66 | 0.999999 |
Target: 5'- cAUUGAUugCAUCg--GCAAgcUCAGUUAa -3' miRNA: 3'- -UAAUUGugGUAGaauUGUU--AGUCAGU- -5' |
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24796 | 5' | -41.5 | NC_005282.1 | + | 53532 | 1.09 | 0.042883 |
Target: 5'- uAUUAACACCAUCUUAACAAUCAGUCAa -3' miRNA: 3'- -UAAUUGUGGUAGAAUUGUUAGUCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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