miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
24796 5' -41.5 NC_005282.1 + 66962 0.66 1
Target:  5'- --cAGCACCAUCUU--CAGUgGGUa- -3'
miRNA:   3'- uaaUUGUGGUAGAAuuGUUAgUCAgu -5'
24796 5' -41.5 NC_005282.1 + 38988 0.66 1
Target:  5'- ---uGCAgCAUCUUGACAAUauaCAGUg- -3'
miRNA:   3'- uaauUGUgGUAGAAUUGUUA---GUCAgu -5'
24796 5' -41.5 NC_005282.1 + 51375 0.66 1
Target:  5'- cUUAcCACCAUaaaguaaCUUAACAAUCccuaAGUCAc -3'
miRNA:   3'- uAAUuGUGGUA-------GAAUUGUUAG----UCAGU- -5'
24796 5' -41.5 NC_005282.1 + 2460 0.66 0.999999
Target:  5'- uUUAACACC-UCaacccguuCAGUCGGUCGu -3'
miRNA:   3'- uAAUUGUGGuAGaauu----GUUAGUCAGU- -5'
24796 5' -41.5 NC_005282.1 + 47847 0.66 0.999999
Target:  5'- cAUUGAUugCAUCg--GCAAgcUCAGUUAa -3'
miRNA:   3'- -UAAUUGugGUAGaauUGUU--AGUCAGU- -5'
24796 5' -41.5 NC_005282.1 + 53532 1.09 0.042883
Target:  5'- uAUUAACACCAUCUUAACAAUCAGUCAa -3'
miRNA:   3'- -UAAUUGUGGUAGAAUUGUUAGUCAGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.