Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24798 | 3' | -44.7 | NC_005282.1 | + | 8766 | 0.68 | 0.996392 |
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Target: 5'- -gGUAAAGcuCUUUAUcUGGGGCUUAc -3' miRNA: 3'- caUAUUUCacGAAAUGuACCCCGAAU- -5' |
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| 24798 | 3' | -44.7 | NC_005282.1 | + | 10433 | 0.68 | 0.996392 |
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Target: 5'- -gGUAAAGcuCUUUAUcUGGGGCUUAc -3' miRNA: 3'- caUAUUUCacGAAAUGuACCCCGAAU- -5' |
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| 24798 | 3' | -44.7 | NC_005282.1 | + | 63099 | 0.69 | 0.994877 |
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Target: 5'- uUAUAAAGgugcucaugGCUUggcUGCAUGGGGUg-- -3' miRNA: 3'- cAUAUUUCa--------CGAA---AUGUACCCCGaau -5' |
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| 24798 | 3' | -44.7 | NC_005282.1 | + | 2287 | 0.77 | 0.788408 |
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Target: 5'- --uUAAAGUGCUUgACAaGGGGCUUu -3' miRNA: 3'- cauAUUUCACGAAaUGUaCCCCGAAu -5' |
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| 24798 | 3' | -44.7 | NC_005282.1 | + | 8621 | 0.82 | 0.52338 |
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Target: 5'- ---aAAAGUaCUUUACAUGGGGCUUAa -3' miRNA: 3'- cauaUUUCAcGAAAUGUACCCCGAAU- -5' |
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| 24798 | 3' | -44.7 | NC_005282.1 | + | 10284 | 1.09 | 0.017036 |
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Target: 5'- aGUAUAAAGUGCUUUACAUGGGGCUUAa -3' miRNA: 3'- -CAUAUUUCACGAAAUGUACCCCGAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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