Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 248 | 5' | -52.1 | AC_000008.1 | + | 15111 | 0.66 | 0.758948 |
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Target: 5'- -cAGCCGCGgcaauGCAGcCGGUGGAg -3' miRNA: 3'- caUUGGCGUaugcuCGUCuGCCACUU- -5' |
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| 248 | 5' | -52.1 | AC_000008.1 | + | 24158 | 0.66 | 0.747903 |
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Target: 5'- gGUAGgCGCGUugGcGGCaucgguggAGGCGGUGGu -3' miRNA: 3'- -CAUUgGCGUAugC-UCG--------UCUGCCACUu -5' |
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| 248 | 5' | -52.1 | AC_000008.1 | + | 24591 | 0.66 | 0.714018 |
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Target: 5'- -cAACCGCAgcCGAGCGGACa----- -3' miRNA: 3'- caUUGGCGUauGCUCGUCUGccacuu -5' |
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| 248 | 5' | -52.1 | AC_000008.1 | + | 1111 | 0.67 | 0.644122 |
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Target: 5'- aGUAAgUGaaaauUAUGGGCAGugGGUGAu -3' miRNA: 3'- -CAUUgGCgu---AUGCUCGUCugCCACUu -5' |
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| 248 | 5' | -52.1 | AC_000008.1 | + | 29944 | 0.68 | 0.597093 |
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Target: 5'- -aGGCUGCAgAUGAGCGuGAgGGUGAc -3' miRNA: 3'- caUUGGCGUaUGCUCGU-CUgCCACUu -5' |
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| 248 | 5' | -52.1 | AC_000008.1 | + | 11153 | 0.69 | 0.573738 |
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Target: 5'- ---uCCGCGguugACGcggcAGCAGAUGGUGAu -3' miRNA: 3'- cauuGGCGUa---UGC----UCGUCUGCCACUu -5' |
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| 248 | 5' | -52.1 | AC_000008.1 | + | 8652 | 0.69 | 0.539139 |
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Target: 5'- cGgcGCCGCGcGCGGGCAGGagcUGGUGc- -3' miRNA: 3'- -CauUGGCGUaUGCUCGUCU---GCCACuu -5' |
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| 248 | 5' | -52.1 | AC_000008.1 | + | 8499 | 0.7 | 0.47669 |
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Target: 5'- -aGGCCGCAUccccgcggcGCGAcuacgguaccgcgcgGCGGGCGGUGGg -3' miRNA: 3'- caUUGGCGUA---------UGCU---------------CGUCUGCCACUu -5' |
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| 248 | 5' | -52.1 | AC_000008.1 | + | 10749 | 0.73 | 0.344127 |
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Target: 5'- gGUAACCGCAUggaucACG-GCGGACGGccgGAu -3' miRNA: 3'- -CAUUGGCGUA-----UGCuCGUCUGCCa--CUu -5' |
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| 248 | 5' | -52.1 | AC_000008.1 | + | 11506 | 1.06 | 0.001772 |
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Target: 5'- gGUAACCGCAUACGAGCAGACGGUGAAc -3' miRNA: 3'- -CAUUGGCGUAUGCUCGUCUGCCACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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