miRNA: 3'- aAAUCUCCCGAagA----UUCCAAUaAUGg -5'
miRNA: 3'- -AAAUCUcCCGAA--GAUUCCAA---------UAAUGG- -5'
miRNA: 3'- -AAAUCUCCCGAAGAUUCCAAUAAUGG- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24800 | 3' | -44 | NC_005282.1 | + | 13690 | 0.69 | 0.998361 |
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Target: 5'- gUUAGAGGGCUagUuagaAGGGUUA-UACa -3' miRNA: 3'- aAAUCUCCCGAagA----UUCCAAUaAUGg -5' |
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| 24800 | 3' | -44 | NC_005282.1 | + | 73228 | 0.7 | 0.996461 |
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Target: 5'- cUUUGGA-GGCUUuaCUAAGGUUccuacacuaGUUACCu -3' miRNA: 3'- -AAAUCUcCCGAA--GAUUCCAA---------UAAUGG- -5' |
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| 24800 | 3' | -44 | NC_005282.1 | + | 26866 | 1.13 | 0.012904 |
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Target: 5'- uUUUAGAGGGCUUCUAAGGUUAUUACCg -3' miRNA: 3'- -AAAUCUCCCGAAGAUUCCAAUAAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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