Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24808 | 3' | -50.3 | NC_005284.1 | + | 22706 | 0.66 | 0.942184 |
Target: 5'- gGGUACggCGUCG-CAuucccuuucacGGAGUACGAg -3' miRNA: 3'- aCCAUGa-GUAGCuGUua---------CCUCGUGCU- -5' |
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24808 | 3' | -50.3 | NC_005284.1 | + | 45851 | 0.66 | 0.937997 |
Target: 5'- cGGg---CAUCGAacCGuuugcuacGUGGAGCGCGAa -3' miRNA: 3'- aCCaugaGUAGCU--GU--------UACCUCGUGCU- -5' |
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24808 | 3' | -50.3 | NC_005284.1 | + | 1619 | 0.66 | 0.937997 |
Target: 5'- uUGcGUGCUCGcgcUCGAC-AUGGcGCGCa- -3' miRNA: 3'- -AC-CAUGAGU---AGCUGuUACCuCGUGcu -5' |
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24808 | 3' | -50.3 | NC_005284.1 | + | 16171 | 0.66 | 0.926701 |
Target: 5'- aUGGgGCUCGgcgCGuCGAUGGcGCugGGc -3' miRNA: 3'- -ACCaUGAGUa--GCuGUUACCuCGugCU- -5' |
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24808 | 3' | -50.3 | NC_005284.1 | + | 20465 | 0.67 | 0.900548 |
Target: 5'- gGGUGCUCccaGUCcuCAAUGGcAGCAgCGAu -3' miRNA: 3'- aCCAUGAG---UAGcuGUUACC-UCGU-GCU- -5' |
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24808 | 3' | -50.3 | NC_005284.1 | + | 25689 | 0.67 | 0.893279 |
Target: 5'- aUGGUGC--GUCGACAAggGGaAGCGgGAu -3' miRNA: 3'- -ACCAUGagUAGCUGUUa-CC-UCGUgCU- -5' |
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24808 | 3' | -50.3 | NC_005284.1 | + | 29314 | 0.67 | 0.885725 |
Target: 5'- -cGUACUgAUCGAuCAGUGGAcGCAUa- -3' miRNA: 3'- acCAUGAgUAGCU-GUUACCU-CGUGcu -5' |
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24808 | 3' | -50.3 | NC_005284.1 | + | 49297 | 0.69 | 0.782903 |
Target: 5'- cGGccGCUCGUCGuucccgaggguacgGCGAUGGuuGGCACGGc -3' miRNA: 3'- aCCa-UGAGUAGC--------------UGUUACC--UCGUGCU- -5' |
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24808 | 3' | -50.3 | NC_005284.1 | + | 16055 | 0.71 | 0.688369 |
Target: 5'- cGGUAaUCGUCGGCAGUa-AGCGCGGg -3' miRNA: 3'- aCCAUgAGUAGCUGUUAccUCGUGCU- -5' |
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24808 | 3' | -50.3 | NC_005284.1 | + | 16501 | 0.72 | 0.654328 |
Target: 5'- aGGUGCUCGacgugaucUCGGCGgggcccaucGUGGGGCugGu -3' miRNA: 3'- aCCAUGAGU--------AGCUGU---------UACCUCGugCu -5' |
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24808 | 3' | -50.3 | NC_005284.1 | + | 6540 | 0.73 | 0.574634 |
Target: 5'- aGGcGCUCAggcgcgCGACAGUGGcuGGUGCGAa -3' miRNA: 3'- aCCaUGAGUa-----GCUGUUACC--UCGUGCU- -5' |
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24808 | 3' | -50.3 | NC_005284.1 | + | 51292 | 0.74 | 0.552175 |
Target: 5'- cGGcGCUUAUUcgGGCAAUGGAGCuCGAg -3' miRNA: 3'- aCCaUGAGUAG--CUGUUACCUCGuGCU- -5' |
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24808 | 3' | -50.3 | NC_005284.1 | + | 12370 | 0.78 | 0.333568 |
Target: 5'- gGGUggGCUCGUUGGCg--GGAGCGCGGg -3' miRNA: 3'- aCCA--UGAGUAGCUGuuaCCUCGUGCU- -5' |
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24808 | 3' | -50.3 | NC_005284.1 | + | 53634 | 1.1 | 0.002824 |
Target: 5'- gUGGUACUCAUCGACAAUGGAGCACGAu -3' miRNA: 3'- -ACCAUGAGUAGCUGUUACCUCGUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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