miRNA: 3'- aaGUGGCGCAGCUaAGCGCGuaa---GCG- -5'
miRNA: 3'- aaGUGGCGCAGCUaa-GCGCGUaaGCG- -5'
miRNA: 3'- aAGUGGC-GCAGCUaAGCGCGU----------AAGCG- -5'
miRNA: 3'- -AAGUGGCGCAGCUAAGCGCGUAAGCG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24810 | 5' | -52.9 | NC_005284.1 | + | 44110 | 0.77 | 0.266263 |
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Target: 5'- -cCGCCGUGUCGA-UCGCGCccgcggCGCg -3' miRNA: 3'- aaGUGGCGCAGCUaAGCGCGuaa---GCG- -5' |
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| 24810 | 5' | -52.9 | NC_005284.1 | + | 46079 | 0.78 | 0.215459 |
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Target: 5'- -gCGCCGCGUCGAgcgCGCGCAc-CGUg -3' miRNA: 3'- aaGUGGCGCAGCUaa-GCGCGUaaGCG- -5' |
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| 24810 | 5' | -52.9 | NC_005284.1 | + | 46899 | 0.81 | 0.15496 |
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Target: 5'- gUCGCCGaCGUCGGcUCGCGCAcugcgaucuuUUCGCc -3' miRNA: 3'- aAGUGGC-GCAGCUaAGCGCGU----------AAGCG- -5' |
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| 24810 | 5' | -52.9 | NC_005284.1 | + | 50781 | 1.1 | 0.001368 |
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Target: 5'- uUUCACCGCGUCGAUUCGCGCAUUCGCg -3' miRNA: 3'- -AAGUGGCGCAGCUAAGCGCGUAAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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