Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24812 | 3' | -51.6 | NC_005284.1 | + | 43071 | 0.7 | 0.664632 |
Target: 5'- -cGCGAUUGAUGcacuGUCGGAucGGUCGGa -3' miRNA: 3'- aaCGCUAGCUGCau--UAGCCU--UCGGCU- -5' |
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24812 | 3' | -51.6 | NC_005284.1 | + | 20678 | 0.72 | 0.539871 |
Target: 5'- -gGCGAUCGGCGUGcaguuuuacgguGUCGGcAcggaaccgggggcGGCCGAg -3' miRNA: 3'- aaCGCUAGCUGCAU------------UAGCC-U-------------UCGGCU- -5' |
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24812 | 3' | -51.6 | NC_005284.1 | + | 6788 | 0.74 | 0.476543 |
Target: 5'- aUGCGGcCGGCGUuugaugcgucGAUCGGcgAGGCCGAg -3' miRNA: 3'- aACGCUaGCUGCA----------UUAGCC--UUCGGCU- -5' |
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24812 | 3' | -51.6 | NC_005284.1 | + | 6853 | 0.75 | 0.41615 |
Target: 5'- -cGCGAUCGAuCGUGugcUCGGAGGgCGGc -3' miRNA: 3'- aaCGCUAGCU-GCAUu--AGCCUUCgGCU- -5' |
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24812 | 3' | -51.6 | NC_005284.1 | + | 48897 | 0.97 | 0.015957 |
Target: 5'- cUUGCG-UCGACGUAAUCGGAAGCCGAu -3' miRNA: 3'- -AACGCuAGCUGCAUUAGCCUUCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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