Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24813 | 3' | -55.7 | NC_005284.1 | + | 22127 | 0.66 | 0.736245 |
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Target: 5'- cCCCGCcgaugCGGGAGaggCGGcagcgUGGCGCUa- -3' miRNA: 3'- -GGGCGa----GCCUUCaa-GCC-----ACUGCGAgc -5' |
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| 24813 | 3' | -55.7 | NC_005284.1 | + | 32973 | 0.66 | 0.725886 |
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Target: 5'- -aCGCUUGGAGGgcggCGGUcacuCGCUCc -3' miRNA: 3'- ggGCGAGCCUUCaa--GCCAcu--GCGAGc -5' |
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| 24813 | 3' | -55.7 | NC_005284.1 | + | 48169 | 0.66 | 0.704911 |
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Target: 5'- gCgCGCUgGcGAAGUgacgcccguugCGGUGACaGCUCGc -3' miRNA: 3'- -GgGCGAgC-CUUCAa----------GCCACUG-CGAGC- -5' |
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| 24813 | 3' | -55.7 | NC_005284.1 | + | 16229 | 0.68 | 0.640677 |
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Target: 5'- uCUCGCUCGGGcuUUCGGgaUGAUcCUCGc -3' miRNA: 3'- -GGGCGAGCCUucAAGCC--ACUGcGAGC- -5' |
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| 24813 | 3' | -55.7 | NC_005284.1 | + | 6866 | 0.7 | 0.523497 |
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Target: 5'- -gUGCUCGGAGGgcggCGGUGAgCGUgaUCGu -3' miRNA: 3'- ggGCGAGCCUUCaa--GCCACU-GCG--AGC- -5' |
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| 24813 | 3' | -55.7 | NC_005284.1 | + | 54373 | 0.71 | 0.443588 |
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Target: 5'- gCCGUgcaagcacCGGggGUgCGGUG-CGCUCGu -3' miRNA: 3'- gGGCGa-------GCCuuCAaGCCACuGCGAGC- -5' |
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| 24813 | 3' | -55.7 | NC_005284.1 | + | 8190 | 0.73 | 0.327588 |
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Target: 5'- gCCGCUCGGGAcGUUCGGcguUGcccacugcgccgccAUGCUCGg -3' miRNA: 3'- gGGCGAGCCUU-CAAGCC---AC--------------UGCGAGC- -5' |
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| 24813 | 3' | -55.7 | NC_005284.1 | + | 10576 | 0.78 | 0.161487 |
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Target: 5'- gCCCaGCUCGGAAGcgCGcGuUGACGCUCu -3' miRNA: 3'- -GGG-CGAGCCUUCaaGC-C-ACUGCGAGc -5' |
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| 24813 | 3' | -55.7 | NC_005284.1 | + | 48716 | 1.1 | 0.001009 |
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Target: 5'- cCCCGCUCGGAAGUUCGGUGACGCUCGc -3' miRNA: 3'- -GGGCGAGCCUUCAAGCCACUGCGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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