Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24815 | 3' | -52 | NC_005284.1 | + | 36815 | 0.66 | 0.866869 |
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Target: 5'- -----cUCGaGCGcuUCGGCCUcGCCCu -3' miRNA: 3'- gcaacaAGC-CGCuuAGUCGGAaCGGG- -5' |
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| 24815 | 3' | -52 | NC_005284.1 | + | 19913 | 0.66 | 0.858549 |
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Target: 5'- uGUUGUguucuaCGGCGAuGUCgAGCCgauCCCa -3' miRNA: 3'- gCAACAa-----GCCGCU-UAG-UCGGaacGGG- -5' |
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| 24815 | 3' | -52 | NC_005284.1 | + | 40857 | 0.67 | 0.84118 |
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Target: 5'- -aUUGUuuUUGGCGGAUCGGa-UUGCCg -3' miRNA: 3'- gcAACA--AGCCGCUUAGUCggAACGGg -5' |
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| 24815 | 3' | -52 | NC_005284.1 | + | 27541 | 0.67 | 0.822897 |
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Target: 5'- gCGggGUUCGGCu-AUUGGCCgcgUGCgCu -3' miRNA: 3'- -GCaaCAAGCCGcuUAGUCGGa--ACGgG- -5' |
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| 24815 | 3' | -52 | NC_005284.1 | + | 37781 | 0.67 | 0.822897 |
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Target: 5'- aCGUUGaucgcgUCGGCGAucGUC-GCCgugUGCgCg -3' miRNA: 3'- -GCAACa-----AGCCGCU--UAGuCGGa--ACGgG- -5' |
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| 24815 | 3' | -52 | NC_005284.1 | + | 54307 | 0.68 | 0.763391 |
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Target: 5'- gGUUGUcgGGUGuuuUCGGCUUUGCgCCg -3' miRNA: 3'- gCAACAagCCGCuu-AGUCGGAACG-GG- -5' |
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| 24815 | 3' | -52 | NC_005284.1 | + | 44011 | 0.69 | 0.709846 |
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Target: 5'- aGUUGUUCGcGUcaGAAagCAGCCgaacGCCCu -3' miRNA: 3'- gCAACAAGC-CG--CUUa-GUCGGaa--CGGG- -5' |
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| 24815 | 3' | -52 | NC_005284.1 | + | 8609 | 0.72 | 0.552737 |
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Target: 5'- ---cGUUCGGCGAGUUcGCCUUccguuucGUCCa -3' miRNA: 3'- gcaaCAAGCCGCUUAGuCGGAA-------CGGG- -5' |
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| 24815 | 3' | -52 | NC_005284.1 | + | 48080 | 1.13 | 0.001287 |
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Target: 5'- gCGUUGUUCGGCGAAUCAGCCUUGCCCu -3' miRNA: 3'- -GCAACAAGCCGCUUAGUCGGAACGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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