Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24818 | 5' | -54.4 | NC_005284.1 | + | 23286 | 0.66 | 0.791549 |
Target: 5'- cGGUGccGAUaUCGGCGUGUaucccGGCGucGUCGAc -3' miRNA: 3'- -CCAC--UUGcAGCCGCGCA-----UCGU--CAGCU- -5' |
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24818 | 5' | -54.4 | NC_005284.1 | + | 54172 | 0.66 | 0.791549 |
Target: 5'- --cGGAU-UCGGUGCGUAGgGGUCa- -3' miRNA: 3'- ccaCUUGcAGCCGCGCAUCgUCAGcu -5' |
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24818 | 5' | -54.4 | NC_005284.1 | + | 39317 | 0.66 | 0.781738 |
Target: 5'- cGGcGAGauaccaGUCGcGCacgaucGCGUAGCAGUCGu -3' miRNA: 3'- -CCaCUUg-----CAGC-CG------CGCAUCGUCAGCu -5' |
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24818 | 5' | -54.4 | NC_005284.1 | + | 12118 | 0.66 | 0.761669 |
Target: 5'- cGGUGucucugaugcCGUCGGCGCGcucGGUGGUgCGGc -3' miRNA: 3'- -CCACuu--------GCAGCCGCGCa--UCGUCA-GCU- -5' |
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24818 | 5' | -54.4 | NC_005284.1 | + | 40396 | 0.66 | 0.759631 |
Target: 5'- --aGcAUGUCGGCGCcgauuuccguGCAGUCGAc -3' miRNA: 3'- ccaCuUGCAGCCGCGcau-------CGUCAGCU- -5' |
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24818 | 5' | -54.4 | NC_005284.1 | + | 21676 | 0.67 | 0.720042 |
Target: 5'- ----cGCGUCGGCG-GUGGCcgccGUCGAc -3' miRNA: 3'- ccacuUGCAGCCGCgCAUCGu---CAGCU- -5' |
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24818 | 5' | -54.4 | NC_005284.1 | + | 29879 | 0.68 | 0.687877 |
Target: 5'- uGGUcGGCGcCGGCGUGUuccaaGGCGaauGUCGAg -3' miRNA: 3'- -CCAcUUGCaGCCGCGCA-----UCGU---CAGCU- -5' |
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24818 | 5' | -54.4 | NC_005284.1 | + | 45652 | 0.68 | 0.677034 |
Target: 5'- uGGcgGcuCGUCGGUGCGcgaGGCGGUgGAa -3' miRNA: 3'- -CCa-CuuGCAGCCGCGCa--UCGUCAgCU- -5' |
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24818 | 5' | -54.4 | NC_005284.1 | + | 16439 | 0.68 | 0.644297 |
Target: 5'- aGGUGGugGU-GGCG-GUGGCAGcagUGAa -3' miRNA: 3'- -CCACUugCAgCCGCgCAUCGUCa--GCU- -5' |
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24818 | 5' | -54.4 | NC_005284.1 | + | 33999 | 0.69 | 0.622399 |
Target: 5'- gGGUGcGGCGagggccgauUCuGCGCGUucgaGGCGGUCGAg -3' miRNA: 3'- -CCAC-UUGC---------AGcCGCGCA----UCGUCAGCU- -5' |
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24818 | 5' | -54.4 | NC_005284.1 | + | 53579 | 0.7 | 0.578778 |
Target: 5'- uGG-GGGCGUgCGGC-UGUGGCGGUCa- -3' miRNA: 3'- -CCaCUUGCA-GCCGcGCAUCGUCAGcu -5' |
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24818 | 5' | -54.4 | NC_005284.1 | + | 24638 | 0.7 | 0.546489 |
Target: 5'- cGGcGAACugaccgucuucaGUCGcGCGCGgcguGCAGUCGAc -3' miRNA: 3'- -CCaCUUG------------CAGC-CGCGCau--CGUCAGCU- -5' |
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24818 | 5' | -54.4 | NC_005284.1 | + | 11870 | 0.71 | 0.514795 |
Target: 5'- -cUGGcGCGUCGGCGUGaAGCGGgCGAu -3' miRNA: 3'- ccACU-UGCAGCCGCGCaUCGUCaGCU- -5' |
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24818 | 5' | -54.4 | NC_005284.1 | + | 18220 | 0.74 | 0.33688 |
Target: 5'- --cGGACGcUCGGCG-GUGGCGGUUGGg -3' miRNA: 3'- ccaCUUGC-AGCCGCgCAUCGUCAGCU- -5' |
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24818 | 5' | -54.4 | NC_005284.1 | + | 17705 | 0.74 | 0.328843 |
Target: 5'- -cUGAAucuCGcCGGCGCGUcgauGGCGGUCGAg -3' miRNA: 3'- ccACUU---GCaGCCGCGCA----UCGUCAGCU- -5' |
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24818 | 5' | -54.4 | NC_005284.1 | + | 47732 | 1.11 | 0.000975 |
Target: 5'- cGGUGAACGUCGGCGCGUAGCAGUCGAu -3' miRNA: 3'- -CCACUUGCAGCCGCGCAUCGUCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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