Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24819 | 3' | -56.1 | NC_005284.1 | + | 42954 | 0.68 | 0.514363 |
Target: 5'- cGCGAGCcgcugcucgagauAGCCGCGCucgcgcgCGaCCUGCu -3' miRNA: 3'- uUGCUUG-------------UCGGCGCGuaa----GC-GGACGu -5' |
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24819 | 3' | -56.1 | NC_005284.1 | + | 5814 | 0.68 | 0.514363 |
Target: 5'- -cCGucCAGCCGCGCAgcaccgcUUCGUCgaUGCAg -3' miRNA: 3'- uuGCuuGUCGGCGCGU-------AAGCGG--ACGU- -5' |
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24819 | 3' | -56.1 | NC_005284.1 | + | 50614 | 0.68 | 0.50482 |
Target: 5'- -uCGAGCAGCCGauucaCGuCGUUCGCCaUGUc -3' miRNA: 3'- uuGCUUGUCGGC-----GC-GUAAGCGG-ACGu -5' |
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24819 | 3' | -56.1 | NC_005284.1 | + | 40278 | 0.66 | 0.636358 |
Target: 5'- cGGCGAugGCuGCUGCGCGUcggaUGUCUGCu -3' miRNA: 3'- -UUGCU--UGuCGGCGCGUAa---GCGGACGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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