miRNA: 3'- uuGCUUgUCGGCGCGuaa------GCGGaCGU- -5'
miRNA: 3'- uugCUUGUCGGCgCGUAAgCGGa--------CGU- -5'
miRNA: 3'- uuGCUUGUCgGCGCGUaAGCGGa-CGU- -5'
miRNA: 3'- -UUGCU-UGuCGGCGCGUA-----AGCGG-ACGU- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24819 | 3' | -56.1 | NC_005284.1 | + | 1276 | 0.67 | 0.558683 |
|
Target: 5'- -uCGAGgGGCCGUGCuucgacaagCGCCgGCAg -3' miRNA: 3'- uuGCUUgUCGGCGCGuaa------GCGGaCGU- -5' |
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| 24819 | 3' | -56.1 | NC_005284.1 | + | 3719 | 0.67 | 0.558683 |
|
Target: 5'- --aGAGCGGCCGgGCGUU-GCCgguucugugGCAg -3' miRNA: 3'- uugCUUGUCGGCgCGUAAgCGGa--------CGU- -5' |
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| 24819 | 3' | -56.1 | NC_005284.1 | + | 21642 | 0.67 | 0.54776 |
|
Target: 5'- -cCGAGCAG-CGUGCGaUCGCCgcGCAc -3' miRNA: 3'- uuGCUUGUCgGCGCGUaAGCGGa-CGU- -5' |
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| 24819 | 3' | -56.1 | NC_005284.1 | + | 33271 | 0.72 | 0.280454 |
|
Target: 5'- gGGCGAuGCuGCCGCGCGUgcuucUUGCCaUGCAu -3' miRNA: 3'- -UUGCU-UGuCGGCGCGUA-----AGCGG-ACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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