Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
24821 | 5' | -56.7 | NC_005284.1 | + | 36857 | 0.66 | 0.629467 |
Target: 5'- gGCGAuugucggcgccuuguCGaGCGUGAUCCUuucgccagccgCUGCGCGGa -3' miRNA: 3'- aCGCU---------------GCaUGCACUAGGG-----------GACGCGUU- -5' |
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24821 | 5' | -56.7 | NC_005284.1 | + | 50131 | 0.66 | 0.592048 |
Target: 5'- cGCGGCGUuCGccauggaccggaUGAUUcguUCCUGCGCAGu -3' miRNA: 3'- aCGCUGCAuGC------------ACUAG---GGGACGCGUU- -5' |
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24821 | 5' | -56.7 | NC_005284.1 | + | 6567 | 0.66 | 0.580008 |
Target: 5'- uUGCGccacaugacagacACGUacGCGUGG-CCgCUGCGCGAg -3' miRNA: 3'- -ACGC-------------UGCA--UGCACUaGGgGACGCGUU- -5' |
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24821 | 5' | -56.7 | NC_005284.1 | + | 30659 | 0.68 | 0.475203 |
Target: 5'- aUGCGugGU-CGUG-UCCCCcuCGCAAa -3' miRNA: 3'- -ACGCugCAuGCACuAGGGGacGCGUU- -5' |
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24821 | 5' | -56.7 | NC_005284.1 | + | 45202 | 0.7 | 0.371004 |
Target: 5'- cGCGGCa-GCGcGAUCgCCCUGCGCu- -3' miRNA: 3'- aCGCUGcaUGCaCUAG-GGGACGCGuu -5' |
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24821 | 5' | -56.7 | NC_005284.1 | + | 45405 | 1.06 | 0.001023 |
Target: 5'- cUGCGACGUACGUGAUCCCCUGCGCAAg -3' miRNA: 3'- -ACGCUGCAUGCACUAGGGGACGCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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