miRNA: 3'- uuACGGCUGacGUAGCGUuCCGa--AAG- -5'
miRNA: 3'- uuaCGGCUGA-CGuaGCGUUCCGAAAG- -5'
miRNA: 3'- -UUACGGCUGACGuag-CGUUC-CGaaag -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24822 | 3' | -52.3 | NC_005284.1 | + | 50168 | 0.66 | 0.791399 |
|
Target: 5'- -cUGCCGAC--CGUCGCA-GGCacgUUCa -3' miRNA: 3'- uuACGGCUGacGUAGCGUuCCGa--AAG- -5' |
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| 24822 | 3' | -52.3 | NC_005284.1 | + | 53498 | 0.7 | 0.566828 |
|
Target: 5'- --cGCCGGUUcGCcgCGCGGGGCUUUUu -3' miRNA: 3'- uuaCGGCUGA-CGuaGCGUUCCGAAAG- -5' |
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| 24822 | 3' | -52.3 | NC_005284.1 | + | 53783 | 0.69 | 0.601173 |
|
Target: 5'- uGAUGCCGGCUGCugcgGCGAGcGCa--- -3' miRNA: 3'- -UUACGGCUGACGuag-CGUUC-CGaaag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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