Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24824 | 5' | -54.7 | NC_005284.1 | + | 24806 | 0.66 | 0.722951 |
Target: 5'- -cAGUCGaGAGCCAgacACUG-GUGCAUCGg -3' miRNA: 3'- guUCGGC-CUUGGU---UGGCuCGCGUAGU- -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 43819 | 0.66 | 0.722951 |
Target: 5'- uUAAG-CGGGACC-GCCGuGCGCcgAUCGa -3' miRNA: 3'- -GUUCgGCCUUGGuUGGCuCGCG--UAGU- -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 37428 | 0.66 | 0.719721 |
Target: 5'- -cGGCCGGGacguuaucgcaGCCGGCCGcgaggaacaucgucGGCGCGaggaUCAg -3' miRNA: 3'- guUCGGCCU-----------UGGUUGGC--------------UCGCGU----AGU- -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 47708 | 0.66 | 0.705637 |
Target: 5'- cCAAGUCGGcaAGCCGAUCGgcgucggugaacgucGGCGCGUa- -3' miRNA: 3'- -GUUCGGCC--UUGGUUGGC---------------UCGCGUAgu -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 27843 | 0.66 | 0.701277 |
Target: 5'- --cGCUGGGagucGCCAgacgacgcGCCGcccGGCGCAUCGc -3' miRNA: 3'- guuCGGCCU----UGGU--------UGGC---UCGCGUAGU- -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 46548 | 0.67 | 0.690331 |
Target: 5'- --uGCC-GAGCCGcaGCgCGAGCGaCAUCAg -3' miRNA: 3'- guuCGGcCUUGGU--UG-GCUCGC-GUAGU- -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 12553 | 0.67 | 0.690331 |
Target: 5'- gCAGGgCGGAACCuuguGgaGAGCGCAa-- -3' miRNA: 3'- -GUUCgGCCUUGGu---UggCUCGCGUagu -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 695 | 0.67 | 0.690331 |
Target: 5'- uCGAuCCGGAGgCGGCCGAGCGaaagcUCGc -3' miRNA: 3'- -GUUcGGCCUUgGUUGGCUCGCgu---AGU- -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 14353 | 0.67 | 0.690331 |
Target: 5'- uCGAGCCGGGucgACUGAUCGAGCuuuUCGa -3' miRNA: 3'- -GUUCGGCCU---UGGUUGGCUCGcguAGU- -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 27375 | 0.67 | 0.679328 |
Target: 5'- cCAAGCCGGAGCUcGCUGAuaccCGgAUCu -3' miRNA: 3'- -GUUCGGCCUUGGuUGGCUc---GCgUAGu -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 42814 | 0.67 | 0.679328 |
Target: 5'- --cGCCGcGAA-CGACaCGAGUGCAUCc -3' miRNA: 3'- guuCGGC-CUUgGUUG-GCUCGCGUAGu -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 9345 | 0.67 | 0.678225 |
Target: 5'- gCGAGUCGGAucaagcgACgGGCgCGAGCGCGa-- -3' miRNA: 3'- -GUUCGGCCU-------UGgUUG-GCUCGCGUagu -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 11842 | 0.67 | 0.657196 |
Target: 5'- uCGAGCagCGGcucgcguugcaGGCCGACUG-GCGCGUCGg -3' miRNA: 3'- -GUUCG--GCC-----------UUGGUUGGCuCGCGUAGU- -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 9512 | 0.67 | 0.646088 |
Target: 5'- uGGGUCGGcGCCcGgCGAGCGCucGUCGa -3' miRNA: 3'- gUUCGGCCuUGGuUgGCUCGCG--UAGU- -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 18837 | 0.68 | 0.623846 |
Target: 5'- gGGGCCGGGcgugcaggGgCAGCCGAGCacGgAUCAa -3' miRNA: 3'- gUUCGGCCU--------UgGUUGGCUCG--CgUAGU- -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 38879 | 0.68 | 0.61273 |
Target: 5'- aAAGCCgGGAAUCAgcACCGAuaGCGCGcgCAc -3' miRNA: 3'- gUUCGG-CCUUGGU--UGGCU--CGCGUa-GU- -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 22547 | 0.68 | 0.601632 |
Target: 5'- cCGGGCCuGAACCuGCU--GCGCAUCGa -3' miRNA: 3'- -GUUCGGcCUUGGuUGGcuCGCGUAGU- -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 38584 | 0.68 | 0.601632 |
Target: 5'- -cGGCCGu--UCGGCCGAGCGCAcgCGg -3' miRNA: 3'- guUCGGCcuuGGUUGGCUCGCGUa-GU- -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 26235 | 0.68 | 0.601632 |
Target: 5'- uGAGUCGGGuCUcuCCGAGCGCGa-- -3' miRNA: 3'- gUUCGGCCUuGGuuGGCUCGCGUagu -5' |
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24824 | 5' | -54.7 | NC_005284.1 | + | 16085 | 0.68 | 0.579525 |
Target: 5'- uGAGCCGGcAGCCGauacguccAUCGAGCcuuucgccGCGUCGa -3' miRNA: 3'- gUUCGGCC-UUGGU--------UGGCUCG--------CGUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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