Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24829 | 5' | -53.4 | NC_005284.1 | + | 3236 | 0.66 | 0.823906 |
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Target: 5'- -gGAUCGCUUCgGAGCGCUcgGAGAgGa -3' miRNA: 3'- ggCUAGCGAGG-CUUGUGGa-CUCUgUa -5' |
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| 24829 | 5' | -53.4 | NC_005284.1 | + | 7967 | 0.67 | 0.78538 |
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Target: 5'- aUCGAUCGgaCCGGGCGCaaGAGGuCGa -3' miRNA: 3'- -GGCUAGCgaGGCUUGUGgaCUCU-GUa -5' |
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| 24829 | 5' | -53.4 | NC_005284.1 | + | 42664 | 0.69 | 0.623846 |
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Target: 5'- aCCGAgCGCgCCGAcgGCAUCaGAGACAc -3' miRNA: 3'- -GGCUaGCGaGGCU--UGUGGaCUCUGUa -5' |
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| 24829 | 5' | -53.4 | NC_005284.1 | + | 21518 | 0.7 | 0.61273 |
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Target: 5'- gCCGG-CGCUugcgUCGAGCACCUGAucGGCGc -3' miRNA: 3'- -GGCUaGCGA----GGCUUGUGGACU--CUGUa -5' |
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| 24829 | 5' | -53.4 | NC_005284.1 | + | 25271 | 0.7 | 0.601632 |
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Target: 5'- ----cCGCUCCGAGCGguuUCUGAGGCGc -3' miRNA: 3'- ggcuaGCGAGGCUUGU---GGACUCUGUa -5' |
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| 24829 | 5' | -53.4 | NC_005284.1 | + | 46444 | 0.7 | 0.601632 |
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Target: 5'- aCCGAUCGCcgucgCCGAAUGCCgccGAG-CGUc -3' miRNA: 3'- -GGCUAGCGa----GGCUUGUGGa--CUCuGUA- -5' |
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| 24829 | 5' | -53.4 | NC_005284.1 | + | 15150 | 0.7 | 0.5972 |
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Target: 5'- uCUGAUCGCUCaGGGCACCgaagaucuucgcgGAGGCu- -3' miRNA: 3'- -GGCUAGCGAGgCUUGUGGa------------CUCUGua -5' |
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| 24829 | 5' | -53.4 | NC_005284.1 | + | 48729 | 0.72 | 0.483127 |
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Target: 5'- uUCGGUgaCGCUCgCGGACGCCcccGAGACAa -3' miRNA: 3'- -GGCUA--GCGAG-GCUUGUGGa--CUCUGUa -5' |
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| 24829 | 5' | -53.4 | NC_005284.1 | + | 42712 | 1.07 | 0.002173 |
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Target: 5'- gCCGAUCGCUCCGAACACCUGAGACAUc -3' miRNA: 3'- -GGCUAGCGAGGCUUGUGGACUCUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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