Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24837 | 5' | -56.2 | NC_005284.1 | + | 16013 | 0.67 | 0.541774 |
Target: 5'- ---aGCUcgaGCAuCCGGUCGGGCGCGa-- -3' miRNA: 3'- aaaaCGA---CGU-GGCUAGCUCGCGCccu -5' |
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24837 | 5' | -56.2 | NC_005284.1 | + | 17749 | 0.66 | 0.596455 |
Target: 5'- ---gGC-GCACCGGcUCGGGCGCuGGc -3' miRNA: 3'- aaaaCGaCGUGGCU-AGCUCGCGcCCu -5' |
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24837 | 5' | -56.2 | NC_005284.1 | + | 53790 | 0.66 | 0.596455 |
Target: 5'- ---gGCUGCugCGG-CGAGCGCa--- -3' miRNA: 3'- aaaaCGACGugGCUaGCUCGCGcccu -5' |
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24837 | 5' | -56.2 | NC_005284.1 | + | 53020 | 0.66 | 0.618602 |
Target: 5'- ---cGCUGCACUGcaaGUCGAugaGCGGGc -3' miRNA: 3'- aaaaCGACGUGGC---UAGCUcg-CGCCCu -5' |
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24837 | 5' | -56.2 | NC_005284.1 | + | 19458 | 0.76 | 0.172208 |
Target: 5'- uUUUUGCggcagGCGCUGAUCG-GUGCGGGc -3' miRNA: 3'- -AAAACGa----CGUGGCUAGCuCGCGCCCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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