Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24838 | 3' | -52.1 | NC_005284.1 | + | 44174 | 0.66 | 0.872877 |
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Target: 5'- -cGcCGACACGAagcggucgaucagUCGCCCGaucucgCGCGAu -3' miRNA: 3'- cuC-GCUGUGCU-------------AGUGGGUaaa---GCGCU- -5' |
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| 24838 | 3' | -52.1 | NC_005284.1 | + | 8352 | 0.66 | 0.865565 |
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Target: 5'- cGGCGACgGCGAUCGgUUcgUUCGCu- -3' miRNA: 3'- cUCGCUG-UGCUAGUgGGuaAAGCGcu -5' |
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| 24838 | 3' | -52.1 | NC_005284.1 | + | 30056 | 0.69 | 0.718917 |
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Target: 5'- gGAGUGcUACGAUCGCCCcggUUCGUu- -3' miRNA: 3'- -CUCGCuGUGCUAGUGGGua-AAGCGcu -5' |
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| 24838 | 3' | -52.1 | NC_005284.1 | + | 26347 | 0.69 | 0.729766 |
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Target: 5'- -cGCGGCACGGUCAacgCCGg--CGCGc -3' miRNA: 3'- cuCGCUGUGCUAGUg--GGUaaaGCGCu -5' |
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| 24838 | 3' | -52.1 | NC_005284.1 | + | 20648 | 0.67 | 0.821375 |
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Target: 5'- cGGCGGC-CGA-CAUCCcaaugucgcaacGUUUCGCGAa -3' miRNA: 3'- cUCGCUGuGCUaGUGGG------------UAAAGCGCU- -5' |
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| 24838 | 3' | -52.1 | NC_005284.1 | + | 28018 | 0.66 | 0.8572 |
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Target: 5'- cGGGCGACAUGGUUgcaGCgCCAgcacCGUGAu -3' miRNA: 3'- -CUCGCUGUGCUAG---UG-GGUaaa-GCGCU- -5' |
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| 24838 | 3' | -52.1 | NC_005284.1 | + | 7660 | 0.66 | 0.8572 |
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Target: 5'- uGGGCGGCGuCGGUCaauggGCUgAUUUCgGCGAc -3' miRNA: 3'- -CUCGCUGU-GCUAG-----UGGgUAAAG-CGCU- -5' |
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| 24838 | 3' | -52.1 | NC_005284.1 | + | 26423 | 0.66 | 0.865565 |
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Target: 5'- uGGGCGACgACGaAUUGCUCAg--UGCGAa -3' miRNA: 3'- -CUCGCUG-UGC-UAGUGGGUaaaGCGCU- -5' |
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| 24838 | 3' | -52.1 | NC_005284.1 | + | 27043 | 0.66 | 0.889113 |
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Target: 5'- gGGGCGGCACGAUCGauuUCC--UUCuCGAc -3' miRNA: 3'- -CUCGCUGUGCUAGU---GGGuaAAGcGCU- -5' |
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| 24838 | 3' | -52.1 | NC_005284.1 | + | 18024 | 0.71 | 0.585075 |
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Target: 5'- cGGCGACACGGUCACgC---UCGcCGAg -3' miRNA: 3'- cUCGCUGUGCUAGUGgGuaaAGC-GCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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