Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24845 | 5' | -53.3 | NC_005284.1 | + | 30689 | 0.7 | 0.57279 |
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Target: 5'- -gGCUUUCUUGCcgacGCGCUCuGCAuaauggACGCGu -3' miRNA: 3'- agCGAAAGGACG----UGCGAG-UGU------UGCGC- -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 28479 | 0.69 | 0.639717 |
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Target: 5'- aUGCUUUCCgcucGCACGCgcCGCAGUGCc -3' miRNA: 3'- aGCGAAAGGa---CGUGCGa-GUGUUGCGc -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 27216 | 0.67 | 0.759921 |
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Target: 5'- uUCGCUUUUUcuaUGUuCGCUUucuGCAGCGCa -3' miRNA: 3'- -AGCGAAAGG---ACGuGCGAG---UGUUGCGc -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 23449 | 0.67 | 0.770276 |
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Target: 5'- gCGCgugUUCUGCGCgucGCUCGcCGACGUu -3' miRNA: 3'- aGCGaa-AGGACGUG---CGAGU-GUUGCGc -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 17673 | 0.71 | 0.53988 |
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Target: 5'- gCGUcuaCCUGCGCGCgaucgugCGCGACGCc -3' miRNA: 3'- aGCGaaaGGACGUGCGa------GUGUUGCGc -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 12762 | 0.66 | 0.828907 |
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Target: 5'- cCGCUcggCCgGCacGCGCUCGCGaagauACGCu -3' miRNA: 3'- aGCGAaa-GGaCG--UGCGAGUGU-----UGCGc -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 9904 | 0.66 | 0.819608 |
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Target: 5'- gCGCg--CCgaaGCGuCGCUCGCGcucGCGCa -3' miRNA: 3'- aGCGaaaGGa--CGU-GCGAGUGU---UGCGc -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 8230 | 0.71 | 0.53988 |
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Target: 5'- cCGC-UUCCUGauguCGCUCGCGcUGCGg -3' miRNA: 3'- aGCGaAAGGACgu--GCGAGUGUuGCGC- -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 6979 | 0.68 | 0.717266 |
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Target: 5'- gUCGUga-CCgcguacaugGCGCGCUCgACAugGCGc -3' miRNA: 3'- -AGCGaaaGGa--------CGUGCGAG-UGUugCGC- -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 4460 | 0.67 | 0.770276 |
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Target: 5'- cUGCUggaggCCgagcaaaaGCugGCUCGCGGCgGCGg -3' miRNA: 3'- aGCGAaa---GGa-------CGugCGAGUGUUG-CGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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