Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24845 | 5' | -53.3 | NC_005284.1 | + | 30689 | 0.7 | 0.57279 |
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Target: 5'- -gGCUUUCUUGCcgacGCGCUCuGCAuaauggACGCGu -3' miRNA: 3'- agCGAAAGGACG----UGCGAG-UGU------UGCGC- -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 17673 | 0.71 | 0.53988 |
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Target: 5'- gCGUcuaCCUGCGCGCgaucgugCGCGACGCc -3' miRNA: 3'- aGCGaaaGGACGUGCGa------GUGUUGCGc -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 8230 | 0.71 | 0.53988 |
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Target: 5'- cCGC-UUCCUGauguCGCUCGCGcUGCGg -3' miRNA: 3'- aGCGaAAGGACgu--GCGAGUGUuGCGC- -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 45305 | 0.71 | 0.507634 |
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Target: 5'- gUCGCcg-CCUGCGuCGC-CGCGACuGCGg -3' miRNA: 3'- -AGCGaaaGGACGU-GCGaGUGUUG-CGC- -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 50157 | 0.72 | 0.46598 |
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Target: 5'- uUCGU--UCCUGCGCaGUcguugCGCAACGCGa -3' miRNA: 3'- -AGCGaaAGGACGUG-CGa----GUGUUGCGC- -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 51473 | 0.72 | 0.435934 |
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Target: 5'- cCGCUcggCCUGCACuaucgGCUCGCAACaGCc -3' miRNA: 3'- aGCGAaa-GGACGUG-----CGAGUGUUG-CGc -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 38984 | 0.76 | 0.274687 |
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Target: 5'- cCGCggg-CUGCGCGCUgGCGACGCa -3' miRNA: 3'- aGCGaaagGACGUGCGAgUGUUGCGc -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 46100 | 0.76 | 0.26767 |
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Target: 5'- cCGUga-CCUGCACGCUUGCGGCGUu -3' miRNA: 3'- aGCGaaaGGACGUGCGAGUGUUGCGc -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 43610 | 0.79 | 0.174225 |
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Target: 5'- cUUGCUUUCC-GCGCGCggcggcCGCGGCGCGa -3' miRNA: 3'- -AGCGAAAGGaCGUGCGa-----GUGUUGCGC- -5' |
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| 24845 | 5' | -53.3 | NC_005284.1 | + | 36665 | 1.1 | 0.001261 |
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Target: 5'- gUCGCUUUCCUGCACGCUCACAACGCGa -3' miRNA: 3'- -AGCGAAAGGACGUGCGAGUGUUGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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