Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24847 | 5' | -62 | NC_005284.1 | + | 42155 | 0.66 | 0.351955 |
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Target: 5'- aCGCaGCGUcguaucGCCG-CCGUGCCCUUg- -3' miRNA: 3'- -GCGgCGCGa-----CGGCaGGUACGGGAGaa -5' |
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| 24847 | 5' | -62 | NC_005284.1 | + | 5415 | 0.67 | 0.298112 |
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Target: 5'- aCGCCucGCGCuUGCCGUCUAUcGUCCg--- -3' miRNA: 3'- -GCGG--CGCG-ACGGCAGGUA-CGGGagaa -5' |
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| 24847 | 5' | -62 | NC_005284.1 | + | 35459 | 0.67 | 0.290963 |
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Target: 5'- uGCCGuUGUcGuuGUCCAUGCCCa--- -3' miRNA: 3'- gCGGC-GCGaCggCAGGUACGGGagaa -5' |
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| 24847 | 5' | -62 | NC_005284.1 | + | 36898 | 0.67 | 0.290963 |
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Target: 5'- cCGCUGCGCggaugcgcccGCCGaUCCGgggccgGCCUUCUUu -3' miRNA: 3'- -GCGGCGCGa---------CGGC-AGGUa-----CGGGAGAA- -5' |
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| 24847 | 5' | -62 | NC_005284.1 | + | 23543 | 0.67 | 0.277069 |
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Target: 5'- gCGuuGUGCaUGCCGUCU-UGCCCaUCg- -3' miRNA: 3'- -GCggCGCG-ACGGCAGGuACGGG-AGaa -5' |
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| 24847 | 5' | -62 | NC_005284.1 | + | 22953 | 0.68 | 0.270323 |
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Target: 5'- gCGCgCGCGUUGCuCGUCUAUGUCUg--- -3' miRNA: 3'- -GCG-GCGCGACG-GCAGGUACGGGagaa -5' |
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| 24847 | 5' | -62 | NC_005284.1 | + | 35598 | 0.69 | 0.232601 |
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Target: 5'- gGCCGCGUUGUCGUUCA--CCUUCg- -3' miRNA: 3'- gCGGCGCGACGGCAGGUacGGGAGaa -5' |
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| 24847 | 5' | -62 | NC_005284.1 | + | 50229 | 0.69 | 0.204639 |
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Target: 5'- uGCgCGCGCUuCCGUCCAUcCCCUUc- -3' miRNA: 3'- gCG-GCGCGAcGGCAGGUAcGGGAGaa -5' |
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| 24847 | 5' | -62 | NC_005284.1 | + | 28459 | 0.75 | 0.073116 |
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Target: 5'- uGCCGCGCUGaagaaUGUCCAUGCUUUCc- -3' miRNA: 3'- gCGGCGCGACg----GCAGGUACGGGAGaa -5' |
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| 24847 | 5' | -62 | NC_005284.1 | + | 35001 | 1.05 | 0.000342 |
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Target: 5'- cCGCCGCGCUGCCGUCCAUGCCCUCUUg -3' miRNA: 3'- -GCGGCGCGACGGCAGGUACGGGAGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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