Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24850 | 3' | -55.8 | NC_005284.1 | + | 9691 | 0.66 | 0.695271 |
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Target: 5'- cCUUGCGaUGCagUCGGCAUugacggcuGGCUCgAUCa -3' miRNA: 3'- cGAACGC-ACG--AGCUGUG--------CCGAGgUGG- -5' |
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| 24850 | 3' | -55.8 | NC_005284.1 | + | 29258 | 0.66 | 0.695271 |
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Target: 5'- aGCgacGCGcGCaCGACgACGGCcgcuccgauUCCACCg -3' miRNA: 3'- -CGaa-CGCaCGaGCUG-UGCCG---------AGGUGG- -5' |
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| 24850 | 3' | -55.8 | NC_005284.1 | + | 31615 | 0.66 | 0.705988 |
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Target: 5'- cCUUGgGUGCgggCGGCGucgagGGCgcauUCCACCu -3' miRNA: 3'- cGAACgCACGa--GCUGUg----CCG----AGGUGG- -5' |
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| 24850 | 3' | -55.8 | NC_005284.1 | + | 49338 | 0.66 | 0.710255 |
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Target: 5'- gGCacGCGUGCgcgacgaccgaacuaUCGGuCGCaGCUUCGCCg -3' miRNA: 3'- -CGaaCGCACG---------------AGCU-GUGcCGAGGUGG- -5' |
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| 24850 | 3' | -55.8 | NC_005284.1 | + | 39515 | 0.66 | 0.716633 |
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Target: 5'- uGCaUGUgGUGCUCGcCGCGGCcgaugugcUUCAUCg -3' miRNA: 3'- -CGaACG-CACGAGCuGUGCCG--------AGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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