Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24850 | 3' | -55.8 | NC_005284.1 | + | 47476 | 0.73 | 0.296646 |
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Target: 5'- gGCUcGCaUGCUCGACGuCGGC-CaCACCg -3' miRNA: 3'- -CGAaCGcACGAGCUGU-GCCGaG-GUGG- -5' |
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| 24850 | 3' | -55.8 | NC_005284.1 | + | 47823 | 0.66 | 0.692044 |
|
Target: 5'- cGCUgcgaUGgGUGCUCGGCauugacgacGCGGaaagcgcacaggaggCCACCg -3' miRNA: 3'- -CGA----ACgCACGAGCUG---------UGCCga-------------GGUGG- -5' |
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| 24850 | 3' | -55.8 | NC_005284.1 | + | 48833 | 0.69 | 0.512007 |
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Target: 5'- ---cGCGUGgCUCGACugGuGCgagCACCg -3' miRNA: 3'- cgaaCGCAC-GAGCUGugC-CGag-GUGG- -5' |
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| 24850 | 3' | -55.8 | NC_005284.1 | + | 49338 | 0.66 | 0.710255 |
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Target: 5'- gGCacGCGUGCgcgacgaccgaacuaUCGGuCGCaGCUUCGCCg -3' miRNA: 3'- -CGaaCGCACG---------------AGCU-GUGcCGAGGUGG- -5' |
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| 24850 | 3' | -55.8 | NC_005284.1 | + | 51396 | 0.66 | 0.695271 |
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Target: 5'- ---aGCGgGCUCGACGCGGCg----- -3' miRNA: 3'- cgaaCGCaCGAGCUGUGCCGaggugg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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