miRNA: 3'- -CGGCUuGUCGUG--GCCGCGCU-CGCCCu -5'
miRNA: 3'- -CGGCu--UGUCGUGGCCGc------GCUCGCCcu -5'
miRNA: 3'- cGGC-UUGUCGUGGCC-GC-GCUCGCCCu -5'
miRNA: 3'- -CGGCUUGUCGUGGCCGCGCUCGCCCU- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24854 | 3' | -60.9 | NC_005284.1 | + | 11864 | 0.74 | 0.140431 |
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Target: 5'- gGCCGAcUGGCGCguCGGCGUGAaGCGGGc -3' miRNA: 3'- -CGGCUuGUCGUG--GCCGCGCU-CGCCCu -5' |
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| 24854 | 3' | -60.9 | NC_005284.1 | + | 21414 | 0.74 | 0.133142 |
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Target: 5'- aGCCGucgGCAGCGCCGGCcgacgaaCGGGCGGu- -3' miRNA: 3'- -CGGCu--UGUCGUGGCCGc------GCUCGCCcu -5' |
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| 24854 | 3' | -60.9 | NC_005284.1 | + | 20826 | 0.82 | 0.036386 |
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Target: 5'- cCCGcAGCAGCGCCGGcCGaCGAGCGGGc -3' miRNA: 3'- cGGC-UUGUCGUGGCC-GC-GCUCGCCCu -5' |
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| 24854 | 3' | -60.9 | NC_005284.1 | + | 29744 | 1.1 | 0.000258 |
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Target: 5'- cGCCGAACAGCACCGGCGCGAGCGGGAu -3' miRNA: 3'- -CGGCUUGUCGUGGCCGCGCUCGCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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