Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24854 | 3' | -60.9 | NC_005284.1 | + | 49355 | 0.68 | 0.308225 |
Target: 5'- aCCGAacuaucggucGCAGCuucGCCGaGCGCGAGCuGGc -3' miRNA: 3'- cGGCU----------UGUCG---UGGC-CGCGCUCGcCCu -5' |
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24854 | 3' | -60.9 | NC_005284.1 | + | 50092 | 0.66 | 0.405514 |
Target: 5'- aGCCGAACAGCAUCGagaaCGUaacGCGGcGAu -3' miRNA: 3'- -CGGCUUGUCGUGGCc---GCGcu-CGCC-CU- -5' |
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24854 | 3' | -60.9 | NC_005284.1 | + | 50891 | 0.68 | 0.308225 |
Target: 5'- uUCGGGCGGC-UCGGCGCauucaucgaGAGCGGaGAu -3' miRNA: 3'- cGGCUUGUCGuGGCCGCG---------CUCGCC-CU- -5' |
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24854 | 3' | -60.9 | NC_005284.1 | + | 54690 | 0.66 | 0.414451 |
Target: 5'- cGCgCGAGCGGUugCGGUGaCGuGGUGGuGGc -3' miRNA: 3'- -CG-GCUUGUCGugGCCGC-GC-UCGCC-CU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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