Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24855 | 5' | -52.7 | NC_005284.1 | + | 31620 | 0.73 | 0.447049 |
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Target: 5'- -----cGAGCGgaACGCGC-CGCGACGAu -3' miRNA: 3'- uuuuauCUCGC--UGCGCGcGUGCUGCU- -5' |
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| 24855 | 5' | -52.7 | NC_005284.1 | + | 6826 | 0.73 | 0.436079 |
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Target: 5'- ----gGGGGCGAUucgcaccgaguugGCGCGCGCGAuCGAu -3' miRNA: 3'- uuuuaUCUCGCUG-------------CGCGCGUGCU-GCU- -5' |
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| 24855 | 5' | -52.7 | NC_005284.1 | + | 50321 | 0.74 | 0.398476 |
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Target: 5'- cGAAGUAuuuGGCGGCGUGC-CGCGGCGAa -3' miRNA: 3'- -UUUUAUc--UCGCUGCGCGcGUGCUGCU- -5' |
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| 24855 | 5' | -52.7 | NC_005284.1 | + | 31431 | 0.74 | 0.389174 |
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Target: 5'- -----cGGGCGACGUGCGCcgcuGCGGCGc -3' miRNA: 3'- uuuuauCUCGCUGCGCGCG----UGCUGCu -5' |
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| 24855 | 5' | -52.7 | NC_005284.1 | + | 9234 | 0.75 | 0.353418 |
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Target: 5'- -----cGGGCGACGCGCcaCGCGGCGGc -3' miRNA: 3'- uuuuauCUCGCUGCGCGc-GUGCUGCU- -5' |
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| 24855 | 5' | -52.7 | NC_005284.1 | + | 19717 | 0.75 | 0.34485 |
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Target: 5'- ----cGGAGCGGCGCGCcucauGCuCGACGGa -3' miRNA: 3'- uuuuaUCUCGCUGCGCG-----CGuGCUGCU- -5' |
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| 24855 | 5' | -52.7 | NC_005284.1 | + | 49315 | 0.75 | 0.342308 |
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Target: 5'- gAGGGUAcGGCGAugguuggcacggcaCGCGUGCGCGACGAc -3' miRNA: 3'- -UUUUAUcUCGCU--------------GCGCGCGUGCUGCU- -5' |
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| 24855 | 5' | -52.7 | NC_005284.1 | + | 47807 | 0.75 | 0.336432 |
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Target: 5'- -----cGAGCGcgccauguACGCGCGuCACGACGAa -3' miRNA: 3'- uuuuauCUCGC--------UGCGCGC-GUGCUGCU- -5' |
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| 24855 | 5' | -52.7 | NC_005284.1 | + | 29250 | 1.07 | 0.002525 |
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Target: 5'- cAAAAUAGAGCGACGCGCGCACGACGAc -3' miRNA: 3'- -UUUUAUCUCGCUGCGCGCGUGCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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