Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24855 | 5' | -52.7 | NC_005284.1 | + | 33130 | 0.68 | 0.709611 |
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Target: 5'- -----cGAcGCGGCGgcauCGUGCGCGGCGAu -3' miRNA: 3'- uuuuauCU-CGCUGC----GCGCGUGCUGCU- -5' |
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| 24855 | 5' | -52.7 | NC_005284.1 | + | 44475 | 0.68 | 0.731451 |
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Target: 5'- -uGAUcGAGCGGCGCcUGCAUGuCGAu -3' miRNA: 3'- uuUUAuCUCGCUGCGcGCGUGCuGCU- -5' |
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| 24855 | 5' | -52.7 | NC_005284.1 | + | 44955 | 0.67 | 0.763396 |
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Target: 5'- --cGUAGGGCGugcccgugauGCGCGCGC-CGAUu- -3' miRNA: 3'- uuuUAUCUCGC----------UGCGCGCGuGCUGcu -5' |
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| 24855 | 5' | -52.7 | NC_005284.1 | + | 38848 | 0.67 | 0.773778 |
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Target: 5'- ------cGGCGuCGCGCGC-CGACGu -3' miRNA: 3'- uuuuaucUCGCuGCGCGCGuGCUGCu -5' |
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| 24855 | 5' | -52.7 | NC_005284.1 | + | 10460 | 0.67 | 0.784006 |
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Target: 5'- ----cAGAGCGAuuucgagugcCGCGgGCAUGGCa- -3' miRNA: 3'- uuuuaUCUCGCU----------GCGCgCGUGCUGcu -5' |
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| 24855 | 5' | -52.7 | NC_005284.1 | + | 3838 | 0.66 | 0.81365 |
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Target: 5'- uGAAGUAGAGaUGA-GCGCuCACGACa- -3' miRNA: 3'- -UUUUAUCUC-GCUgCGCGcGUGCUGcu -5' |
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| 24855 | 5' | -52.7 | NC_005284.1 | + | 9907 | 0.66 | 0.832432 |
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Target: 5'- -----uGAGCGACgGCGUGCGcCGuauCGAg -3' miRNA: 3'- uuuuauCUCGCUG-CGCGCGU-GCu--GCU- -5' |
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| 24855 | 5' | -52.7 | NC_005284.1 | + | 23346 | 0.66 | 0.850329 |
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Target: 5'- ----gGGGGCccGGCGCGCcGCAgCGGCGc -3' miRNA: 3'- uuuuaUCUCG--CUGCGCG-CGU-GCUGCu -5' |
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| 24855 | 5' | -52.7 | NC_005284.1 | + | 29250 | 1.07 | 0.002525 |
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Target: 5'- cAAAAUAGAGCGACGCGCGCACGACGAc -3' miRNA: 3'- -UUUUAUCUCGCUGCGCGCGUGCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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