Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24862 | 5' | -65.8 | NC_005284.1 | + | 47538 | 0.66 | 0.245329 |
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Target: 5'- uGCCGaCCGuGCgcuccgcauugaagcUGCUCGACuGCUGCAu -3' miRNA: 3'- gCGGCcGGC-CG---------------ACGAGCUGcCGGCGU- -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 33228 | 0.66 | 0.248913 |
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Target: 5'- aCGCCcGCgCGGcCUGCUCGuCGGCaaGCc -3' miRNA: 3'- -GCGGcCG-GCC-GACGAGCuGCCGg-CGu -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 39500 | 0.66 | 0.248913 |
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Target: 5'- gGCgCGGuuGGUcagugcauguggUGCUCGccGCGGCCGa- -3' miRNA: 3'- gCG-GCCggCCG------------ACGAGC--UGCCGGCgu -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 21571 | 0.66 | 0.248913 |
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Target: 5'- gGCCGcGCgGGC-GUUCGACGcGCaGCGg -3' miRNA: 3'- gCGGC-CGgCCGaCGAGCUGC-CGgCGU- -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 13603 | 0.66 | 0.26117 |
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Target: 5'- gGCUGGCCaGCU-CggggCGACGGUCGaCGg -3' miRNA: 3'- gCGGCCGGcCGAcGa---GCUGCCGGC-GU- -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 15960 | 0.66 | 0.26117 |
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Target: 5'- aCGCCGGCCcacguucGCcgUGUUCaACGGCCGg- -3' miRNA: 3'- -GCGGCCGGc------CG--ACGAGcUGCCGGCgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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