Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24862 | 5' | -65.8 | NC_005284.1 | + | 46181 | 0.68 | 0.180027 |
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Target: 5'- aGCCGGCCGucgacgagagaccGC-GCaugCGGCGGUCGUAu -3' miRNA: 3'- gCGGCCGGC-------------CGaCGa--GCUGCCGGCGU- -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 46479 | 0.68 | 0.167259 |
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Target: 5'- aCGCUGGCguuaGCUGCUCGugcAUGGCCGaCGc -3' miRNA: 3'- -GCGGCCGgc--CGACGAGC---UGCCGGC-GU- -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 47538 | 0.66 | 0.245329 |
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Target: 5'- uGCCGaCCGuGCgcuccgcauugaagcUGCUCGACuGCUGCAu -3' miRNA: 3'- gCGGCcGGC-CG---------------ACGAGCUGcCGGCGU- -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 47734 | 0.7 | 0.119509 |
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Target: 5'- gGCCGuGCuCGGCgGUaCGGCGGCgCGCGu -3' miRNA: 3'- gCGGC-CG-GCCGaCGaGCUGCCG-GCGU- -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 49327 | 0.67 | 0.225827 |
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Target: 5'- aGCC-GCCGGCuUGCUCGACuaaUGCAc -3' miRNA: 3'- gCGGcCGGCCG-ACGAGCUGccgGCGU- -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 53780 | 0.7 | 0.129255 |
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Target: 5'- uGCUGauGCCGGCUGCUgCGGCGaG-CGCAu -3' miRNA: 3'- gCGGC--CGGCCGACGA-GCUGC-CgGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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