Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24862 | 5' | -65.8 | NC_005284.1 | + | 12762 | 0.68 | 0.189806 |
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Target: 5'- cCGCuCGGCCGGCacgcGCUCGcgaagauacGCuGUCGCAc -3' miRNA: 3'- -GCG-GCCGGCCGa---CGAGC---------UGcCGGCGU- -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 8619 | 0.67 | 0.209726 |
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Target: 5'- uCGaCGGCCGGCucguauuucaaUGCggugcgCGACGGCgggCGCAc -3' miRNA: 3'- -GCgGCCGGCCG-----------ACGa-----GCUGCCG---GCGU- -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 7011 | 0.68 | 0.189806 |
|
Target: 5'- gCGCUcGCCgGGCUGaCU-GGCGGCCGUu -3' miRNA: 3'- -GCGGcCGG-CCGAC-GAgCUGCCGGCGu -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 4922 | 0.72 | 0.091758 |
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Target: 5'- gCGCCGGCa--CUGCaaucguauaUCGACGGCCGUg -3' miRNA: 3'- -GCGGCCGgccGACG---------AGCUGCCGGCGu -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 4170 | 0.71 | 0.104496 |
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Target: 5'- --aUGGCgaacgacgugaauCGGCUGCUCGACGGCCuCAa -3' miRNA: 3'- gcgGCCG-------------GCCGACGAGCUGCCGGcGU- -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 889 | 0.68 | 0.180482 |
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Target: 5'- gCGCaGGCCaaGGCaaGCgaCGACGGCCGCc -3' miRNA: 3'- -GCGgCCGG--CCGa-CGa-GCUGCCGGCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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