Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24862 | 5' | -65.8 | NC_005284.1 | + | 43603 | 0.67 | 0.225827 |
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Target: 5'- uCGaaGGCUuGCUuuccGCgcgCGGCGGCCGCGg -3' miRNA: 3'- -GCggCCGGcCGA----CGa--GCUGCCGGCGU- -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 49327 | 0.67 | 0.225827 |
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Target: 5'- aGCC-GCCGGCuUGCUCGACuaaUGCAc -3' miRNA: 3'- gCGGcCGGCCG-ACGAGCUGccgGCGU- -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 27958 | 0.67 | 0.21498 |
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Target: 5'- gGUCGGCCGGCauacGCUCGAgcaacgcaUGGCucucgagauCGCAu -3' miRNA: 3'- gCGGCCGGCCGa---CGAGCU--------GCCG---------GCGU- -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 19027 | 0.67 | 0.209726 |
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Target: 5'- cCGCCGGCaCGaaGCUGaucaUCGACGGCgacgagaacaGCAu -3' miRNA: 3'- -GCGGCCG-GC--CGACg---AGCUGCCGg---------CGU- -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 15681 | 0.67 | 0.209726 |
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Target: 5'- aCGCUGGCCuuCUGUcCuuCGGCCGCAu -3' miRNA: 3'- -GCGGCCGGccGACGaGcuGCCGGCGU- -5' |
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| 24862 | 5' | -65.8 | NC_005284.1 | + | 24122 | 1.07 | 0.000147 |
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Target: 5'- cCGCCGGCCGGCUGCUCGACGGCCGCAc -3' miRNA: 3'- -GCGGCCGGCCGACGAGCUGCCGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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