Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24863 | 3' | -54.7 | NC_005284.1 | + | 26915 | 0.66 | 0.803972 |
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Target: 5'- uGCGGgacugauuUCGGcGAUGcgCCGGGCgggcGUCGu -3' miRNA: 3'- gCGCU--------AGCC-CUGCuaGGCCUGa---UAGC- -5' |
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| 24863 | 3' | -54.7 | NC_005284.1 | + | 50826 | 0.66 | 0.794472 |
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Target: 5'- uGCGAuggccgUUGGGugGggCCGGGCguuuUCc -3' miRNA: 3'- gCGCU------AGCCCugCuaGGCCUGau--AGc -5' |
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| 24863 | 3' | -54.7 | NC_005284.1 | + | 14625 | 0.66 | 0.774988 |
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Target: 5'- aCGUGG-CGGuucGCGAgauagCCGGAUUGUCGu -3' miRNA: 3'- -GCGCUaGCCc--UGCUa----GGCCUGAUAGC- -5' |
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| 24863 | 3' | -54.7 | NC_005284.1 | + | 12195 | 0.67 | 0.712389 |
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Target: 5'- uCGCGAcgggCGGGGCGGUguggggaCCGGGCacGUCc -3' miRNA: 3'- -GCGCUa---GCCCUGCUA-------GGCCUGa-UAGc -5' |
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| 24863 | 3' | -54.7 | NC_005284.1 | + | 22888 | 0.68 | 0.66316 |
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Target: 5'- cCGCGAgcugaucauuuucagUUGGGACGAUgcggcgcagccagUCGGGCUGUUc -3' miRNA: 3'- -GCGCU---------------AGCCCUGCUA-------------GGCCUGAUAGc -5' |
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| 24863 | 3' | -54.7 | NC_005284.1 | + | 30784 | 0.68 | 0.649095 |
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Target: 5'- uGCGAUCGcaccGACGAuagUCCGGAUcGUCc -3' miRNA: 3'- gCGCUAGCc---CUGCU---AGGCCUGaUAGc -5' |
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| 24863 | 3' | -54.7 | NC_005284.1 | + | 16238 | 0.69 | 0.638258 |
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Target: 5'- gGCuuUCGGGAUGAUCCucgccuacGGcaucACUAUCGg -3' miRNA: 3'- gCGcuAGCCCUGCUAGG--------CC----UGAUAGC- -5' |
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| 24863 | 3' | -54.7 | NC_005284.1 | + | 569 | 0.72 | 0.440557 |
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Target: 5'- cCGCGcaacauuUCGGcGACGAUUCGGGCaaagAUCGa -3' miRNA: 3'- -GCGCu------AGCC-CUGCUAGGCCUGa---UAGC- -5' |
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| 24863 | 3' | -54.7 | NC_005284.1 | + | 23988 | 1.09 | 0.001552 |
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Target: 5'- cCGCGAUCGGGACGAUCCGGACUAUCGu -3' miRNA: 3'- -GCGCUAGCCCUGCUAGGCCUGAUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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