Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
24864 | 3' | -50.7 | NC_005284.1 | + | 16566 | 0.66 | 0.929383 |
Target: 5'- --cUCGACGGCACgc-CGAUCCagaacGCGg -3' miRNA: 3'- aaaAGCUGUUGUGacaGCUAGGc----CGC- -5' |
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24864 | 3' | -50.7 | NC_005284.1 | + | 20853 | 0.66 | 0.923437 |
Target: 5'- --aUCGGCGugAUcGUCGGUauucucaCGGCGa -3' miRNA: 3'- aaaAGCUGUugUGaCAGCUAg------GCCGC- -5' |
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24864 | 3' | -50.7 | NC_005284.1 | + | 44123 | 0.66 | 0.917196 |
Target: 5'- ---aCGACAaguGCGCUGUgaCGAUCgaGGCa -3' miRNA: 3'- aaaaGCUGU---UGUGACA--GCUAGg-CCGc -5' |
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24864 | 3' | -50.7 | NC_005284.1 | + | 31315 | 0.66 | 0.90383 |
Target: 5'- --aUCGACAGugUUGUCGAaaacgUCGGCGa -3' miRNA: 3'- aaaAGCUGUUguGACAGCUa----GGCCGC- -5' |
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24864 | 3' | -50.7 | NC_005284.1 | + | 47815 | 0.66 | 0.91066 |
Target: 5'- --cUUGGCGGCGCUG-CGAUgggugcUCGGCa -3' miRNA: 3'- aaaAGCUGUUGUGACaGCUA------GGCCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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