Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24864 | 3' | -50.7 | NC_005284.1 | + | 38001 | 0.7 | 0.758817 |
Target: 5'- -gUUCGACGuguccugACGCUGUaGcgCCGGCa -3' miRNA: 3'- aaAAGCUGU-------UGUGACAgCuaGGCCGc -5' |
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24864 | 3' | -50.7 | NC_005284.1 | + | 428 | 0.71 | 0.648884 |
Target: 5'- gUUUCGgcGCAAUGCUGgggcUCGAcCCGGCGa -3' miRNA: 3'- aAAAGC--UGUUGUGAC----AGCUaGGCCGC- -5' |
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24864 | 3' | -50.7 | NC_005284.1 | + | 5371 | 0.74 | 0.510844 |
Target: 5'- --gUCGACGACuucgaaaagaacauGgUGUCGAUCCGuGCGg -3' miRNA: 3'- aaaAGCUGUUG--------------UgACAGCUAGGC-CGC- -5' |
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24864 | 3' | -50.7 | NC_005284.1 | + | 18502 | 0.75 | 0.430701 |
Target: 5'- --cUCGGCGugAC-GUCGAuUCCGGCGu -3' miRNA: 3'- aaaAGCUGUugUGaCAGCU-AGGCCGC- -5' |
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24864 | 3' | -50.7 | NC_005284.1 | + | 23477 | 1.07 | 0.004213 |
Target: 5'- gUUUUCGACAACACUGUCGAUCCGGCGu -3' miRNA: 3'- -AAAAGCUGUUGUGACAGCUAGGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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