Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24864 | 3' | -50.7 | NC_005284.1 | + | 7356 | 0.69 | 0.790007 |
Target: 5'- --gUCGACcgaucucGACGCgGcCGAUCUGGCGu -3' miRNA: 3'- aaaAGCUG-------UUGUGaCaGCUAGGCCGC- -5' |
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24864 | 3' | -50.7 | NC_005284.1 | + | 5371 | 0.74 | 0.510844 |
Target: 5'- --gUCGACGACuucgaaaagaacauGgUGUCGAUCCGuGCGg -3' miRNA: 3'- aaaAGCUGUUG--------------UgACAGCUAGGC-CGC- -5' |
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24864 | 3' | -50.7 | NC_005284.1 | + | 4766 | 0.68 | 0.839223 |
Target: 5'- --aUCGAguACACcGUCGAaaCCGGCu -3' miRNA: 3'- aaaAGCUguUGUGaCAGCUa-GGCCGc -5' |
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24864 | 3' | -50.7 | NC_005284.1 | + | 4418 | 0.69 | 0.810915 |
Target: 5'- --gUCGACGA-GCUGUUGAUCaagcagGGCGa -3' miRNA: 3'- aaaAGCUGUUgUGACAGCUAGg-----CCGC- -5' |
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24864 | 3' | -50.7 | NC_005284.1 | + | 428 | 0.71 | 0.648884 |
Target: 5'- gUUUCGgcGCAAUGCUGgggcUCGAcCCGGCGa -3' miRNA: 3'- aAAAGC--UGUUGUGAC----AGCUaGGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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