Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24866 | 5' | -51.8 | NC_005284.1 | + | 43957 | 0.66 | 0.876632 |
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Target: 5'- cGAGAGCUugcGGGAGCAGaaggagCUcgcgggaugaCGCCCUCg -3' miRNA: 3'- -UUCUUGA---CCUUCGUUaa----GA----------GCGGGGG- -5' |
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| 24866 | 5' | -51.8 | NC_005284.1 | + | 21640 | 0.66 | 0.876632 |
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Target: 5'- -uGAGCUGGcAGGUAuugcagC-CGCCCCUc -3' miRNA: 3'- uuCUUGACC-UUCGUuaa---GaGCGGGGG- -5' |
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| 24866 | 5' | -51.8 | NC_005284.1 | + | 48553 | 0.66 | 0.84246 |
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Target: 5'- -cGGACUGGAuaaacgguGCAGaaCUUGCgCCCa -3' miRNA: 3'- uuCUUGACCUu-------CGUUaaGAGCGgGGG- -5' |
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| 24866 | 5' | -51.8 | NC_005284.1 | + | 10774 | 0.67 | 0.794481 |
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Target: 5'- --uAACUGaAAGCGA-UUUCGCCCCUu -3' miRNA: 3'- uucUUGACcUUCGUUaAGAGCGGGGG- -5' |
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| 24866 | 5' | -51.8 | NC_005284.1 | + | 34330 | 0.67 | 0.784291 |
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Target: 5'- uGAGGACUGGGAGCAcc-CUCcaccguccaGCCCguuCCa -3' miRNA: 3'- -UUCUUGACCUUCGUuaaGAG---------CGGG---GG- -5' |
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| 24866 | 5' | -51.8 | NC_005284.1 | + | 5091 | 0.69 | 0.720012 |
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Target: 5'- cAAGAuccguCUGGcacuguugcAAGCAGUUCUCGCCgaauuUCCg -3' miRNA: 3'- -UUCUu----GACC---------UUCGUUAAGAGCGG-----GGG- -5' |
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| 24866 | 5' | -51.8 | NC_005284.1 | + | 31673 | 0.7 | 0.628357 |
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Target: 5'- -uGGACUGGAcgcugucGGCccgucguUUC-CGCCCCCa -3' miRNA: 3'- uuCUUGACCU-------UCGuu-----AAGaGCGGGGG- -5' |
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| 24866 | 5' | -51.8 | NC_005284.1 | + | 22965 | 1.09 | 0.001811 |
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Target: 5'- gAAGAACUGGAAGCAAUUCUCGCCCCCc -3' miRNA: 3'- -UUCUUGACCUUCGUUAAGAGCGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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