Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24871 | 5' | -56.2 | NC_005284.1 | + | 37055 | 0.72 | 0.267774 |
Target: 5'- -cGGuGCGcCUGCGCCUGCGACGuGCAg -3' miRNA: 3'- aaCC-UGCuGAUGUGGGCGUUGC-CGUa -5' |
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24871 | 5' | -56.2 | NC_005284.1 | + | 8440 | 0.75 | 0.179436 |
Target: 5'- aUGGucCGGCga-GCCCGCGACGGCGg -3' miRNA: 3'- aACCu-GCUGaugUGGGCGUUGCCGUa -5' |
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24871 | 5' | -56.2 | NC_005284.1 | + | 9591 | 0.77 | 0.13587 |
Target: 5'- -aGGGCGAUcgcGCugCCGCGGCGGCGa -3' miRNA: 3'- aaCCUGCUGa--UGugGGCGUUGCCGUa -5' |
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24871 | 5' | -56.2 | NC_005284.1 | + | 323 | 0.81 | 0.070122 |
Target: 5'- -aGGACGAUUugACUCGCAACGGCc- -3' miRNA: 3'- aaCCUGCUGAugUGGGCGUUGCCGua -5' |
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24871 | 5' | -56.2 | NC_005284.1 | + | 9234 | 0.82 | 0.060562 |
Target: 5'- -cGGGCGAC-GCGCCaCGCGGCGGCAUa -3' miRNA: 3'- aaCCUGCUGaUGUGG-GCGUUGCCGUA- -5' |
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24871 | 5' | -56.2 | NC_005284.1 | + | 20256 | 1.03 | 0.001797 |
Target: 5'- gUUGGACGACUACACCCGCAACGGCAUc -3' miRNA: 3'- -AACCUGCUGAUGUGGGCGUUGCCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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