Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24871 | 5' | -56.2 | NC_005284.1 | + | 44115 | 0.67 | 0.560655 |
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Target: 5'- gUGu-CGAUcGCGCCCGCGGCGcGCAc -3' miRNA: 3'- aACcuGCUGaUGUGGGCGUUGC-CGUa -5' |
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| 24871 | 5' | -56.2 | NC_005284.1 | + | 25544 | 0.67 | 0.560655 |
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Target: 5'- aUGGA-GAUUACGCCgGCAAUGGa-- -3' miRNA: 3'- aACCUgCUGAUGUGGgCGUUGCCgua -5' |
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| 24871 | 5' | -56.2 | NC_005284.1 | + | 33108 | 0.66 | 0.582677 |
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Target: 5'- gUUGGuCGACggcggcCACCgccgaCGCGGCGGCAUc -3' miRNA: 3'- -AACCuGCUGau----GUGG-----GCGUUGCCGUA- -5' |
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| 24871 | 5' | -56.2 | NC_005284.1 | + | 5389 | 0.66 | 0.604868 |
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Target: 5'- -cGGAguuCGGCUGCuuGCCCGU-GCGGCGc -3' miRNA: 3'- aaCCU---GCUGAUG--UGGGCGuUGCCGUa -5' |
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| 24871 | 5' | -56.2 | NC_005284.1 | + | 19121 | 0.66 | 0.627155 |
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Target: 5'- aUGaGACGACacCGCgCGCgAACGGCAUc -3' miRNA: 3'- aAC-CUGCUGauGUGgGCG-UUGCCGUA- -5' |
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| 24871 | 5' | -56.2 | NC_005284.1 | + | 20256 | 1.03 | 0.001797 |
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Target: 5'- gUUGGACGACUACACCCGCAACGGCAUc -3' miRNA: 3'- -AACCUGCUGAUGUGGGCGUUGCCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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