Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24871 | 5' | -56.2 | NC_005284.1 | + | 32981 | 0.69 | 0.435372 |
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Target: 5'- -aGGGCGGCggucacuCGCuCCGCAGCGGgCGa -3' miRNA: 3'- aaCCUGCUGau-----GUG-GGCGUUGCC-GUa -5' |
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| 24871 | 5' | -56.2 | NC_005284.1 | + | 42764 | 0.69 | 0.442254 |
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Target: 5'- gUGGGCGAUUGCgaccacgcacaacaACCuCGCGcCGGCAUu -3' miRNA: 3'- aACCUGCUGAUG--------------UGG-GCGUuGCCGUA- -5' |
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| 24871 | 5' | -56.2 | NC_005284.1 | + | 12965 | 0.69 | 0.445222 |
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Target: 5'- cUUGGGCGA--GCACCCaGCGgaagugGCGGCGa -3' miRNA: 3'- -AACCUGCUgaUGUGGG-CGU------UGCCGUa -5' |
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| 24871 | 5' | -56.2 | NC_005284.1 | + | 21233 | 0.68 | 0.50676 |
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Target: 5'- -gGGGCGcACaUAUcaGCUCGCGGCGGCGg -3' miRNA: 3'- aaCCUGC-UG-AUG--UGGGCGUUGCCGUa -5' |
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| 24871 | 5' | -56.2 | NC_005284.1 | + | 17888 | 0.67 | 0.517377 |
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Target: 5'- -cGGAuCGGCgggcGCAUCCGCGcaGCGGCu- -3' miRNA: 3'- aaCCU-GCUGa---UGUGGGCGU--UGCCGua -5' |
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| 24871 | 5' | -56.2 | NC_005284.1 | + | 323 | 0.81 | 0.070122 |
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Target: 5'- -aGGACGAUUugACUCGCAACGGCc- -3' miRNA: 3'- aaCCUGCUGAugUGGGCGUUGCCGua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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