Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24873 | 5' | -51.1 | NC_005284.1 | + | 19956 | 0.7 | 0.763391 |
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Target: 5'- ----aUCGACGcCGGUGCCGGGcuGGCUu -3' miRNA: 3'- ccuuaGGCUGCaGCUACGGCUU--UCGA- -5' |
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| 24873 | 5' | -51.1 | NC_005284.1 | + | 677 | 0.7 | 0.752914 |
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Target: 5'- aGGAuuUCCGAUGgaaguUCGAU-CCGGAGGCg -3' miRNA: 3'- -CCUu-AGGCUGC-----AGCUAcGGCUUUCGa -5' |
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| 24873 | 5' | -51.1 | NC_005284.1 | + | 9208 | 0.7 | 0.720762 |
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Target: 5'- -uGAUCaGGCGUCGAUaGCCGAGuGCg -3' miRNA: 3'- ccUUAGgCUGCAGCUA-CGGCUUuCGa -5' |
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| 24873 | 5' | -51.1 | NC_005284.1 | + | 47505 | 0.71 | 0.669974 |
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Target: 5'- aGGAugcuaucgccgucguGUUCGGCGUCGAggUGCCGAccGUg -3' miRNA: 3'- -CCU---------------UAGGCUGCAGCU--ACGGCUuuCGa -5' |
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| 24873 | 5' | -51.1 | NC_005284.1 | + | 37212 | 0.72 | 0.631866 |
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Target: 5'- uGAcgCCGAUGUCGGUGuuGAccgacAGGCc -3' miRNA: 3'- cCUuaGGCUGCAGCUACggCU-----UUCGa -5' |
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| 24873 | 5' | -51.1 | NC_005284.1 | + | 35362 | 0.73 | 0.587062 |
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Target: 5'- uGAAUgCCGAU-UCGAUGCCGAAcGCUu -3' miRNA: 3'- cCUUA-GGCUGcAGCUACGGCUUuCGA- -5' |
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| 24873 | 5' | -51.1 | NC_005284.1 | + | 12108 | 0.73 | 0.542879 |
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Target: 5'- uGGGAUUCGGCGgugucucUGAUGCCGucGGCg -3' miRNA: 3'- -CCUUAGGCUGCa------GCUACGGCuuUCGa -5' |
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| 24873 | 5' | -51.1 | NC_005284.1 | + | 46623 | 0.74 | 0.510471 |
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Target: 5'- cGGG--UCGACGUCGAgcuUGCCGAauucGAGCg -3' miRNA: 3'- -CCUuaGGCUGCAGCU---ACGGCU----UUCGa -5' |
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| 24873 | 5' | -51.1 | NC_005284.1 | + | 39410 | 0.75 | 0.438361 |
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Target: 5'- cGAAUUCGcaccaguCGUCGAUGCCGAucGAGCc -3' miRNA: 3'- cCUUAGGCu------GCAGCUACGGCU--UUCGa -5' |
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| 24873 | 5' | -51.1 | NC_005284.1 | + | 19457 | 1.11 | 0.002229 |
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Target: 5'- uGGAAUCCGACGUCGAUGCCGAAAGCUa -3' miRNA: 3'- -CCUUAGGCUGCAGCUACGGCUUUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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