Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24874 | 5' | -50.9 | NC_005284.1 | + | 11087 | 0.73 | 0.552928 |
Target: 5'- --gGUGGUCGcGAAGGCGcUUGAGGAg -3' miRNA: 3'- ccgCGCUAGCuCUUCCGCuAACUUCU- -5' |
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24874 | 5' | -50.9 | NC_005284.1 | + | 19027 | 0.74 | 0.520159 |
Target: 5'- aGGCgGCGAUCGccGAGGGCGAUaUGgcGGu -3' miRNA: 3'- -CCG-CGCUAGCu-CUUCCGCUA-ACuuCU- -5' |
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24874 | 5' | -50.9 | NC_005284.1 | + | 15058 | 0.75 | 0.477724 |
Target: 5'- uGGaCGaacaGAUCaAGAAGGCGAUUGAGGc -3' miRNA: 3'- -CC-GCg---CUAGcUCUUCCGCUAACUUCu -5' |
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24874 | 5' | -50.9 | NC_005284.1 | + | 36364 | 0.76 | 0.398476 |
Target: 5'- cGGcCGCGAcgauguUCGAGAAGGCag-UGGAGAu -3' miRNA: 3'- -CC-GCGCU------AGCUCUUCCGcuaACUUCU- -5' |
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24874 | 5' | -50.9 | NC_005284.1 | + | 49260 | 0.78 | 0.328164 |
Target: 5'- cGCGCGAUC-AGGAGGCGAcgUGAcGAa -3' miRNA: 3'- cCGCGCUAGcUCUUCCGCUa-ACUuCU- -5' |
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24874 | 5' | -50.9 | NC_005284.1 | + | 18447 | 1.13 | 0.001749 |
Target: 5'- cGGCGCGAUCGAGAAGGCGAUUGAAGAa -3' miRNA: 3'- -CCGCGCUAGCUCUUCCGCUAACUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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